caspase-3 antibody staining

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caspase-3 antibody staining

Postby Shang » Tue May 27, 2008 4:26 pm

Hi, everyone, do you have any advice/tricks for caspase-3 antibody staining? I have tried several protocols and they didn't work well. Essentially the background signal is too strong. Thanks very much!
Shang
 
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Joined: Tue May 27, 2008 4:23 pm

Re: caspase-3 antibody staining

Postby jkeyser » Tue Sep 30, 2008 1:36 am

Why do you need to see the caspases ? their activity may be easier to detect ...

Have you tried pre-stained kits from suppliers ?
If you need colorimetric kit for caspase 3, and the fluorescent version of the kit.
Hope this helps (i m a bit of a noob)...
jkeyser
 
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Joined: Mon Sep 29, 2008 5:14 am


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