Anti beta-actin loading control

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Anti beta-actin loading control

Postby djjosep » Mon Jul 07, 2008 5:54 pm


I've been trying the anti beta-actin loading control (Sigma) for Western blotting with Drosophila melanogaster protein extracts. The problem is that I tried lots of concentrations (1:250, 1:1500, 1:2500, 1:5000, 1:7500, 1:10000) and I didn't get any result. I used secondary antibody anti-mouse-POD at 1:5000, which works perfectly with the primary antibody I use to detect my protein of interest and with anti-tubulin loading control.

Can anybody help me, please? If someone had the same problem, a piece of advice will be much appreciated.

Thank you very much!
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