Aberration Dmel\Df(2L)A400

General Information
Symbol	Dmel\Df(2L)A400	Species	D. melanogaster
Name	Deficiency (2L) Aaron	FlyBase ID	FBab0001413
Feature type	chromosomal_deletion

Computed Breakpoints include	35A1-35A4;35C1-35C3
Deleted segment	35A1--35C3
Sequence coordinates
Member of large scale dataset(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Aberration
Cytological Order
Progenitor
Mutagen	X ray (Ashburner, 1982, Fossett et al., 1994)
Class of aberration (relative to progenitor)	chromosomal_deletion (Gubb et al., 1990, Roote et al., 1996)
Breakpoints	35A1-35A4;35B10 (Gubb et al., 1990, Roote et al., 1996)
Causes alleles
Carries alleles
Transposon Insertions
Formalized genetic data	Pgant35A << bk1 << elB << stc << bk2 << rd
Genetic mapping information
Comments
Comments on Cytology
	Adhr status unknown. (Roote, 2002.1.15) Left limit of break 1 from polytene analysis (FBrf0051930) Right limit of break 1 from inclusion of elB (FBrf0051916) Left limit of break 2 from inclusion of TfIIS (FBrf0073088) Right limit of break 2 from non-inclusion of esg (FBrf0073088)
Sequence Crossreferences
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name
Gene Deletion & Duplication Data
Genes Deleted / Disrupted
Complementation Data
Completely deleted / disrupted	Adh (Ashburner et al., 1982, Ashburner et al., 1982, Ashburner, 1982, Fossett et al., 1994, Roote et al., 1996) ck (Ashburner et al., 1982, Ashburner et al., 1982, Ashburner, 1982, Fossett et al., 1994) dyn-p25 (Ashburner et al., 1982, Ashburner, 1982, Roote, 2010.1.4) elA (Ashburner et al., 1982, Ashburner et al., 1982, Davis et al., 1990, Fossett et al., 1994, Roote et al., 1996, Davis et al., 1997) elB (Davis et al., 1990, Fossett et al., 1994, Roote et al., 1996, Davis et al., 1997) l(2)35Bc (Ashburner et al., 1982, Ashburner, 1982) l(2)35Bd (Ashburner et al., 1982, Ashburner, 1982) l(2)35Be (Ashburner et al., 1982, Ashburner, 1982) l(2)35Bg (Ashburner et al., 1982, Ashburner et al., 1982, Ashburner, 1982) mol (Ashburner et al., 1982, Ashburner et al., 1982, Ashburner, 1982, Fossett et al., 1994, Roote et al., 1996) noc (Ashburner et al., 1982, Ashburner et al., 1982, Davis et al., 1990, Fossett et al., 1994, Roote et al., 1996, Davis et al., 1997) osp (Ashburner et al., 1982, Ashburner et al., 1982, Fossett et al., 1994, Roote et al., 1996) pburs (Ashburner et al., 1982, Ashburner et al., 1982, Davis et al., 1990, Roote et al., 1996, Davis et al., 1997) ppk (Ainsley et al., 2003) stc (Ashburner et al., 1982, Ashburner, 1982, Fossett et al., 1994, Roote et al., 1996) Su(H) (Ashburner et al., 1982, Ashburner, 1982) TfIIS (Fossett et al., 1994)
Molecular Data
Genes NOT Deleted / Disrupted
Complementation Data
	Ance (Ashburner et al., 1982, Fossett et al., 1994) Arpc1 (Ashburner et al., 1982) b (Ashburner et al., 1982) Ca-α1D (Fossett et al., 1994) Cul-3 (Ashburner et al., 1982, Ashburner et al., 1982, Ashburner, 1982, Fossett et al., 1994) CycE (Ashburner et al., 1982, Ashburner et al., 1982, Ashburner, 1982, Fossett et al., 1994) DNApol-γ35 (Ashburner et al., 1982) esg (Fossett et al., 1994) kuz (Fossett et al., 1994) l(2)34Db (Ashburner et al., 1982, Fossett et al., 1994) l(2)34Fc (Ashburner et al., 1982, Fossett et al., 1994, Ashburner et al., 1982, Roote et al., 1996) l(2)34Fd (Davis et al., 1990, Ashburner et al., 1982, Roote et al., 1996) l(2)35Cc (Fossett et al., 1994) l(2)35De (Fossett et al., 1994) lace (Ashburner et al., 1982, Ashburner et al., 1982, Ashburner, 1982, Fossett et al., 1994) mRpL4 (Fossett et al., 1994) ms(2)34Fe (Roote et al., 1996, Davis et al., 1990) NimA (Ashburner et al., 1982) NimC3 (Ashburner et al., 1982, Fossett et al., 1994) Pgant35A (Flores and Engels, 1999, Ashburner et al., 1982, Ashburner et al., 1982, Davis et al., 1990, Fossett et al., 1994, Roote et al., 1996) rd (Ashburner et al., 1982, Ashburner et al., 1982, Ashburner, 1982) rk (Ashburner et al., 1982, Fossett et al., 1994) RpII33 (Ashburner et al., 1982, Fossett et al., 1994) sna (Ashburner et al., 1982, Ashburner et al., 1982, Ashburner, 1982, Fossett et al., 1994) Sos (Ashburner et al., 1982) stc (Ashburner et al., 1982) tam (Ashburner et al., 1982) wb (Fossett et al., 1994, Ashburner et al., 1982) wek (Fossett et al., 1994) wor (Ashburner et al., 1982, Ashburner et al., 1982, Ashburner, 1982, Fossett et al., 1994)
Molecular Data
Genes Duplicated
Complementation Data
Molecular Data
Genes NOT Duplicated
Complementation Data
Molecular Data
Related Comments

Phenotypic Data
In combination with other aberrations	Df(2L)A400/Df(2L)b88h49 animals are viable as larvae and adults, with no morphological or behavioural abnormalities easily detected during routine culture. Df(2L)A400/Df(2L)b88h49 larvae do not have defects in multiple dendritic neuron morphology. The larvae are normal in their response to external touch with an eyelash or single hair and show a normal response to four different odours in an olfactory taxis assay. The larvae are indistinguishable from wild type in their ability to respond to a hot probe (pain assay). Df(2L)A400/Df(2L)b88h49 third instar larvae move with a decreased number of stops or turns compared to wild-type larvae, resulting in an extended straight or slightly arching contour trail and a total contour trail area nearly twice that of wild-type larvae. Crawling speed during a linear locomotion burst is increased in the mutant larvae compared to wild type. The mutant larvae complete a full contraction wave cycle in only 1.11 seconds compared to 1.94 seconds in wild-type larvae, due to a more rapid progression of the contraction wave from posterior to anterior and a significant decrease in the length of the pause period between contraction waves (Ainsley et al., 2003) Df(2L)b83d29a/Df(2L)A400 is pburs^-. (Davis, 2001.4.23) Viable in combination with Df(2L)b88h49. (Ashburner et al., 1999) Df(2L)A400/Df(2L)b83d29a animals do not usually survive to adulthood, rare escapers have crippled legs and show "elbow" and pburs phenotypes. Df(2L)A400/Df(2L)b84a2 animals die as pharate adults with crippled legs. (Davis et al., 1997) Weakly suppresses the zeste phenotype in a z¹ w^11E4; Tp(1;2)TE35B homologue. (Gubb et al., 1990)
NOT in combination with other aberrations	The Df(2L)A400 chromosome does not act as a dominant suppressor of telomeric silencing (assayed using the effect of the chromosome on the eye colour phenotype of flies carrying "P{w^var}KR3-2", a stable "brown-red" variant of the P{3'WP-2,w^var}2Lt insertion). (Mason et al., 2004) Chromosome acts as a weak trans-suppressor of a physical interaction between tandemly duplicated copies of w⁺, an interaction that is required for the suppression of genes by z¹. (Gubb et al., 1990)
Stocks ( 3 )
Bloomington	6266 T(2;3;4)CA4, Df(2L)A400, b¹ cn¹ bw¹/CyO, Adh^nB 25128 Df(2L)A400, T(2;3;4)CA4, b¹ cn¹ bw¹/CyO, Adh^nB
Kyoto	108708 T(2;3;4)CA4, Df(2L)A400, b¹ cn¹ bw¹/CyO, Adh^nB
Notes on Origin
Discoverer	Aaron.
	selected as ADH null on 1-penten-3-ol. Induced with, but genetically separable from T(2;3;4)CA4.
Balancer / Genotype Variants of the Aberration

Separable Components

Other Comments
	Reported to delete Adhr in error; see FBrf0141768 for correction. (Roote et al., 1996) Chromosome also carries T(2;3;4)CA4. (Ashburner et al., 1982)
Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	A400 (Fossett et al., 1994) Df(2L)A400 (Roote, 2010.1.4)
Name Synonym	Deficiency (2L) Aaron
Secondary FlyBase IDs

References ( 20 )
Research paper	Mason et al., 2004, Genetics 168(3): 1353--1370 A deficiency screen for dominant suppressors of telomeric silencing in Drosophila. [FBrf0180277] Ainsley et al., 2003, Curr. Biol. 13(17): 1557--1563 Enhanced locomotion caused by loss of the Drosophila DEG/ENaC protein Pickpocket1. [FBrf0162002] Ashburner et al., 1999, Genetics 153(1): 179--219 An exploration of the sequence of a 2.9-Mb region of the genome of Drosophila melanogaster. The Adh region. [FBrf0111326] Flores and Engels, 1999, Proc. Natl. Acad. Sci. U.S.A. 96(6): 2964--2969 Microsatellite instability in Drosophila spellchecker1 (MutS homolog) mutants. [FBrf0108178] Davis et al., 1997, Hereditas 126(1): 67--75 Genetic and phenotypic analysis of the genes of the elbow-no-ocelli region of chromosome 2L of Drosophila melanogaster. [FBrf0093413] Roote et al., 1996, D. I. S. 77: 55--65 Aberrations in the l(2)34Fc - l(2)35Bb region of chromosome arm 2L. [FBrf0091422] Fossett et al., 1994, Mutat. Res. 307(1): 213--222 The influence of large deletions on the mutation frequency induced by tritiated water and X-radiation in male Drosophila melanogaster post-meiotic germ cells. [FBrf0073088] Ashburner et al., 1990, Genetics 126: 679--694 The genetics of a small autosomal region of Drosophila melanogaster containing the structural gene for alcohol dehydrogenase. [FBrf0051973] Davis et al., 1990, Genetics 126: 105--119 The molecular analysis of the el-noc complex of Drosophila melanogaster. [FBrf0051916] Gubb et al., 1990, Genetics 126: 167--176 A novel transvection phenomenon affecting the white gene of Drosophila melanogaster. [FBrf0051930] Ashburner et al., 1983, Genetics 104: 405--431 The genetics of a small autosomal region of Drosophila melanogaster containing the structural gene for alcohol dehydrogenase. [FBrf0039455] Ashburner et al., 1982, Genetics 102: 421--435 The genetics of a small autosomal region of Drosophila melanogaster, including the structural gene for alcohol dehydrogenase. [FBrf0038050] Ashburner, 1982, Genetics 101: 447--459 The genetics of a small autosomal region of Drosophila melanogaster containing the structural gene for Alcohol dehydrogenase. [FBrf0038053] Ashburner et al., 1982, Genetics 102: 401--420 The genetics of a small chromosome region of Drosophila melanogaster containing the structural gene for Alcohol dehydrogenase. [FBrf0038047] Aaron, 1979, Mutat. Res. 63: 127--137 X-ray-induced mutations affecting the level of the enzyme alcohol dehydrogenase in Drosophila melanogaster: frequency and genetic analysis of null-enzyme mutants. [FBrf0033674]
Personal communication to FlyBase	Roote, 2010.1.4, Analysis of Zuker alleles. Analysis of Zuker alleles. [FBrf0209649] Roote, 2002.1.15, Adhr status unknown. Adhr status unknown. [FBrf0141768] Davis, 2001.4.23, FlyBase error report for CG15284 and CG3474 on Mon Apr 23 02:12:23 2001. FlyBase error report for CG15284 and CG3474 on Mon Apr 23 02:12:23 2001. [FBrf0138570]
FlyBase analysis	FlyBase, 2007, En masse symbol-based assigment of Aberration Class with respect to wild type. En masse symbol-based assigment of Aberration Class with respect to wild type. [FBrf0191808]
Book	Lindsley and Zimm, 1992, The Genome of Drosophila melanogaster. The Genome of Drosophila melanogaster. [FBrf0066905]