Df(2L)x528/Df(2L)Gpdh1-A embryos show a loss of neuroblasts NB1-1, NB2-5 and NB2-4. This loss is much more pronounced in odd-numbered than even-numbered abdominal segments. However, the loss is similar to mid mutants, suggesting that the it is deletion of the mid gene and not the H15 gene that is causing the phenotype.
Df(2L)Gpdh1-A/Df(2L)x528 larvae show a loss of denticle belts; denticle rows 1 to 5 are frequently lost in odd-numbered segments, whereas even-numbered segments show milder defects. Df(2L)H15-x4/Df(2L)Gpdh1-A larvae show a weak loss of denticles phenotype.