Aberration Dmel\Df(2L)ast2

General Information
Symbol	Dmel\Df(2L)ast2	Species	D. melanogaster
Name	Deficiency (2L) asteroid	FlyBase ID	FBab0001695
Feature type	chromosomal_deletion	Created / Updated	2006-08-22/2006-08-22

Formalized genetic data	Gsc << bk1 << ds << l(2)04111 << bk2 << Su(dx)
Sequence coordinates
Deleted segment	21D1--22B3
Duplicated segment
Computed Breakpoints include	21D1-21D2;22B2-22B3
Breakpoints Inherited
Nature of the Aberration
Cytological Order
Progenitor
Mutagen	γ ray (Roberts et al., 1985)
Class of aberration (relative to progenitor)	chromosomal_deletion (Pauli et al., 1995, de Belle et al., 1989, Harbecke and Lengyel, 1995, Hopmann et al., 1996, Roberts et al., 1985, Adler et al., 1998, Fostier et al., 1998)
Breakpoints	21D1-21D2;22B2-22B3 (Roberts et al., 1985, de Belle et al., 1989, Pauli et al., 1995, Harbecke and Lengyel, 1995, Adler et al., 1998) 22A2-22A3;22B2-22B3 (Hopmann et al., 1996) 22D1-22D2;22B2-22B3 (Fostier et al., 1998)
Causes alleles
Carries alleles
Transposon Insertions
Genetic mapping information
Comments
Comments on Cytology
	All limits from polytene analysis (FBrf0042627)
Molecularly Mapped Breakpoints

Sequence Crossreferences
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name
Gene Deletion & Duplication Data
Genes Deleted / Disrupted
Complementation Data
Completely deleted / disrupted	cpb (Hopmann et al., 1996, Delalle et al., 2005) RFeSP (Spradling et al., 1999) ast (Roberts et al., 1985, Kolodkin et al., 1994) S (BDGP Project Members, 1994-1999, Kolodkin et al., 1994, Begemann et al., 1995, Kania et al., 1995, Harbecke and Lengyel, 1995, Clegg et al., 1997, Spradling et al., 1999, Thibault et al., 2004) Hsp60B (Spradling et al., 1999) dock (BDGP Project Members, 1994-1999, Garrity et al., 1996, Spradling et al., 1999) dbe (Spradling et al., 1999) ds (Adler et al., 1998) capt (BDGP Project Members, 1994-1999, Spradling et al., 1999) l(2)01855 (Spradling et al., 1999) l(2)10685 (Spradling et al., 1999) chinmo (BDGP Project Members, 1994-1999, Spradling et al., 1999)
Molecular Data
Genes NOT Deleted / Disrupted
Complementation Data
	Rab5 (Spradling et al., 1999) Su(dx) (Fostier et al., 1998) ex (Spradling et al., 1999) Gsc (BDGP Project Members, 1994-1999, Spradling et al., 1999) Nle (Spradling et al., 1999) lwr (Spradling et al., 1999) α-Adaptin (Spradling et al., 1999) for (de Belle et al., 1989) l(2)k09624 (Spradling et al., 1999) l(2)02321 (BDGP Project Members, 1994-1999)
Molecular Data
Genes Duplicated
Complementation Data
Molecular Data
Genes NOT Duplicated
Complementation Data
Molecular Data
Related Comments

Phenotypic Data
In combination with other aberrations
NOT in combination with other aberrations	Df(2L)ast2 in combination with a pair of introgressions from D.simulans spanning 30F1-31E7 to 35D7-36A14 Dsim\Int(2L)S and 21A1 to 22D1--23A2 Dsim\Int(2L)D Dsim\Int(2L)D produces semi-sterile male flies. These flies are female sterile. (Sawamura et al., 2000) Deficient embryos show a variably penetrant mutant midgut phenotype: no constrictions form. (Bilder and Scott, 1995) Does not cause unconditional lethality in hybrid females when heterozygous with D.simulans chromosome. (Coyne et al., 1998) Double heterozygous with T(Y;2)Elp show a reduced eye phenotype much more pronounced than for double heterozygotes for T(Y;2)Elp and S¹. (Kolodkin et al., 1994) Eye phenotype is dominantly enhanced by ast^K6. (Kotarski et al., 1998) Heterozygosity for this deletion has no effect on the mutant ovarian phenotype of ovo^D2. (Pauli et al., 1995) Homozygous embryos have head defects, do not complete germband retraction and tracheae are disconnected. (Harbecke and Lengyel, 1995) No second site non-complementing phenotype with zip^Ebr and zip^mhc-c6.1. (Halsell and Kiehart, 1998) Shows no maternal enhancement of dpp^hr4. (Nicholls and Gelbart, 1998) Suppressor of the dosage dependent (two or more copies of P{sev-svp1} or P{sev-svp2}) transformation of cone cells into R7 photoreceptors and at a lower frequency R7 cells into outer photoreceptors. (Begemann et al., 1995) Wild-type clones made in the ovaries of a mutant female, are significantly larger than clones of heterozygous mutant cells. This size difference is greater in clones initiated at 48 hours after egg laying (AEL) than 2 hours AEL. (Extavour and Garcia-Bellido, 2001)
Position Effect Variegation Data

Stocks ( 2 )
Bloomington	3084 Df(2L)ast2/SM1
Kyoto	106828 Df(2L)ast2/SM1
Notes on Origin
Discoverer

Balancer / Genotype Variants of the Aberration

Separable Components

Other Comments
	Df(2L)ast1 as reported is necessarily haplolethal due to deletion of dpp. The original isolation may have included a transposition of some or all of the deleted region. Many studies that have reported haploviabiity of Df(2L)ast1 have probably in fact used a mis-labelled stock of Df(2L)ast2. This stock was originally labelled "Df(2L)ast1" but is assumed to be "Df(2L)ast2" based on its complementation behaviour. (Spradling et al., 1999) The Df(2L)ast2 chromosome may act as a dominant suppressor of telomeric silencing (assayed using the effect of the chromosome on the eye colour phenotype of flies carrying "P{w^var}KR3-2", a stable "brown-red" variant of the P{3'WP-2,w^var}2Lt insertion), but the eye colour phenotype in the presence of Df(2L)ast2 overlaps the eye colour phenotype in a wild-type background so it cannot be unequivocally demonstrated that the deficiency chromosome uncovers a suppressor of telomeric silencing. (Mason et al., 2004)
Synonyms & Secondary IDs ( 9 )
Reported As
Symbol Synonym	ast2 (Yamaguchi et al., 1999) Df(2)ast2 (Hopmann et al., 1996) Df(2L)ast (Fostier et al., 1998) Df(2L)Ast2 (Wills et al., 2002) Df(2L)AST2 (Wills et al., 2002) Df(2L)ast-2 (Extavour and Garcia-Bellido, 2001, Roberts et al., 1985, Pauli et al., 1995, Schupbach and Wieschaus, 1989) Df(2L)ast2 (Crest et al., 2007, Zhu et al., 2006) Df(2L)ast² (Albin and Davis, 2004, Zhang et al., 1999, Garrity et al., 1996)
Name Synonym	Deficiency (2L) asteroid
Secondary FlyBase IDs

References ( 50 )

Generate a list of	All references relating to this aberration ( 50 )

List References by type	Research paper ( 46 ) Personal communication to FlyBase ( 1 ) FlyBase analysis ( 1 ) Computer file ( 1 ) Book ( 1 )
Recent research papers ( 2 )
	Crest et al., 2007, Genetics 175(2): 567--584 Onset of the DNA replication checkpoint in the early Drosophila embryo. [FBrf0194644] Zhu et al., 2006, Cell 127(2): 409--422 Gradients of the Drosophila Chinmo BTB-zinc finger protein govern neuronal temporal identity. [FBrf0194848] Show all research papers ...