21D1-21D2;21E2
21D1-21D2;21E1-21E2
bk1 << l(2)21Dg << l(2)21Dd << bk2
Df(2L)ast4 heterozygotes have a missing-bract phenotype (17% of bracts in the tibia and 78% in the basitarsus of the second leg remain).
The Df(2L)ast4 chromosome acts as a dominant moderate suppressor of telomeric silencing (assayed using the effect of the chromosome on the eye colour phenotype of flies carrying "P{wvar}KR3-2", a stable "brown-red" variant of the P{3'WP-2,wvar}2Lt insertion), but this is assumed to be a false positive result (the suppressor may not be within the bounds of the deficient region) because the 2L TAS array on the chromosome is missing (as assayed by in situ hybridization) and it has previously been shown (FBrf0137248, FBrf0158986) that partial or complete deficiency of the 2L TAS array on the homologue suppresses silencing of brown-red variants of P{3'WP-2,wvar}2Lt. In addition, the deficiency chromosome fails to complement lethal mutations of l(2)gl and deficiencies that are mutant for l(2)gl are often also deficient for the 2L TAS array (FBrf0137248).
Limits of break 1 from polytene analysis (FBrf0042627) Left limit of break 2 from inclusion of ast (FBrf0042627) Right limit of break 2 from polytene analysis (FBrf0042627)