Aberration Dmel\Df(2R)gsb

General Information
Symbol	Dmel\Df(2R)gsb	Species	D. melanogaster
Name		FlyBase ID	FBab0001966
Feature type	chromosomal_deletion
Also Known As	Df(2R)IIX62, Df(2R)gsb^IIX62

Computed Breakpoints include	60E9;60F1
Deleted segment	60E9--60F1
Sequence coordinates
Member of large scale dataset(s)
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Aberration
Cytological Order
Progenitor
Mutagen	ethyl methanesulfonate (Gotwals and Fristrom, 1991)
Class of aberration (relative to progenitor)	chromosomal_deletion (Cote et al., 1987, Germeraad et al., 1992)
Breakpoints	60E9-60E10;60F1-60F2 (Germeraad et al., 1992) 60E9;60F1 (Cote et al., 1987)
Causes alleles	gsb^IIX62 zip^IIX62 (Blake et al., 1998, Young et al., 1993)
Carries alleles
Transposon Insertions
Formalized genetic data	bk1 hits zip << Tkr << bk2 << Kr
Genetic mapping information
Comments	Deficiency breakpoint within the zip locus. (Young et al., 1993) Proximal breakpoint at -55 to -49 kb and distal breakpoint at +40 to +42.5 kb (Cote et al., 1987), with the proximal breakpoint of Df(2R)SB1 approximately at 0.
Comments on Cytology
	Ref: Nusslein-Volhard et al., 1984, Roux Arch. dev. Biol. 193: 267--282 (Germeraad et al., 1992) Left limit of break 1 from polytene analysis (FBrf0046110) Right limit of break 1 from inclusion of zip (FBrf0045941) Left limit of break 2 from inclusion of Tkr (FBrf0046110) Right limit of break 2 from polytene analysis (FBrf0046110)
Sequence Crossreferences
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name
Gene Deletion & Duplication Data
Genes Deleted / Disrupted
Complementation Data
Completely deleted / disrupted	CG16778 (Cote et al., 1987, Duman-Scheel et al., 1997) gol (Cote et al., 1987, Duman-Scheel et al., 1997) gsb (Nusslein-Volhard et al., 1984, Tearle and Nusslein-Volhard, 1987, Cote et al., 1987, Gutjahr et al., 1993, BDGP Project Members, 1994-1999, Li and Noll, 1994, Li and Noll, 1994, van den Heuvel et al., 1993, Bhat, 1996, Matsuzaki and Saigo, 1996, Jagla et al., 1997, Duman-Scheel et al., 1997) gsb-n (Nusslein-Volhard et al., 1984, Tearle and Nusslein-Volhard, 1987, Cote et al., 1987, Gutjahr et al., 1993, Li and Noll, 1994, Li and Noll, 1994, Bhat, 1996, Matsuzaki and Saigo, 1996, Jagla et al., 1997, Duman-Scheel et al., 1997) uzip (Duman-Scheel et al., 1997) zip (Tearle and Nusslein-Volhard, 1987, Cote et al., 1987, Young et al., 1993)
Partially deleted / disrupted	zip (Okumura et al., 2010)
Molecular Data
Genes NOT Deleted / Disrupted
Complementation Data
	bap (Azpiazu et al., 1996) CG3511 (Edgar et al., 2005) Kr (Tearle and Nusslein-Volhard, 1987, BDGP Project Members, 1994-1999, Gray et al., 1994) rib (Blake et al., 1998) RpL19 (Hart et al., 1993) SG13 (Ma et al., 2009) zip (Gotwals and Fristrom, 1991, Duman-Scheel et al., 1997)
Molecular Data
Genes Duplicated
Complementation Data
Molecular Data
Genes NOT Duplicated
Complementation Data
Molecular Data
Related Comments

Phenotypic Data
In combination with other aberrations	Ecol\lacZ^Y72 staining of Df(2R)gsb/Df(2R)Kr10 mutants suggests aCC and pCC neurons are not duplicated (contrary to results of FBrf0049834). Df(2R)gsb/Df(2R)Kr10 mutants exhibit duplicated RP2 neurons and lack U and CQ neurons, phenotype can be rescued by gsb^+t20.1 (though rescue of U and CQ neurons is incomplete). Posterior commissures are missing or reduced, phenotype varies between segments but no segment is wild type. (Duman-Scheel et al., 1997) No effect on In(1)w^m4h position-effect variegation. (Wustmann et al., 1989) Df(2R)gsb/Df(2R)SB1 embryos express gsb but not zip (Cote et al., 1987).
NOT in combination with other aberrations	In the embryonic motor system of mutant animals exhibit frequently fused ISN and SN nerve roots but the respective dendritic fields do not appear to intermingle. (Landgraf et al., 2003) Mutant embryos show an increase in apoptotic nuclei in the anterior portion of the segment similar to that seen in wg^l-12 and arm⁸, though the increase in cell death is restricted to the ventral and ventrolateral surface of the embryo. There is also a slight in crease in cell death in the ventral en-expressing cells. (Pazdera et al., 1998) Neuroblast NB5-2 is transformed into MP2. (Bhat and Schedl, 1997) Homozygotes exhibit duplication of the RP2 lineage due to NB5-3 changing to NB4-2 identity. Simultaneous elimination of gsb rescues the RP2 lineage in 70% of ptc^H84 hemisegments. Ectopic expression of P{HS-wg} causes triplication of the RP2 lineage. (Bhat, 1996) Cuticular phenotype is rescued by the construct P{gsb-prd}. (Li and Noll, 1994) Defective in gonad assembly. (Warrior, 1994) P{gsb⁺} rescues the cuticle phenotype of Df(2R)gsb homozygotes or heterozygotes with Df(2R)Kr10. (Gutjahr et al., 1993)
Stocks ( 2 )
Bloomington	2528 Df(2R)gsb, cn¹ bw¹ zip^IIX62 gsb^IIX62/CyO
Kyoto	107384 y^* w^*; Df(2R)gsb, cn¹ bw¹ zip^IIX62 gsb^IIX62/SM1, sp²
Notes on Origin
Discoverer

Balancer / Genotype Variants of the Aberration

Separable Components

Other Comments
	The Df(2R)gsb chromosome may act as a dominant suppressor of telomeric silencing (assayed using the effect of the chromosome on the eye colour phenotype of flies carrying "P{w^var}KR3-2", a stable "brown-red" variant of the P{3'WP-2,w^var}2Lt insertion), but the eye colour phenotype in the presence of Df(2R)gsb overlaps the eye colour phenotype in a wild-type background so it cannot be unequivocally demonstrated that the deficiency chromosome uncovers a suppressor of telomeric silencing. In addition, any suppression is a false positive result (the suppressor is not within the bounds of the deficient region) because the region of the deficiency is covered by a combination of nonsuppressing deficiencies and deficiencies with suppressors that map to the 2L tip. (Mason et al., 2004) Df(2R)ES1/Df(2R)gsb embryos fail to complete dorsal closure. (Young et al., 1993) Distal breakpoint maps to position +40 to +42.5 and the proximal breakpoint to position -55 to -49 on the map of the 60E9-F1 interval. (Cote et al., 1987)
Synonyms & Secondary IDs ( 10 )
Reported As
Symbol Synonym	BL-2528 (Edgar et al., 2005) Df2R(gsb) (Landgraf et al., 2003) Df(2R)11X62 (Hart et al., 1993) Df(2R)gsb (Ma et al., 2009, Okumura et al., 2010) Df(2R)gsb^IIX62 (Jagla et al., 1997, Li and Noll, 1994, Li and Noll, 1994, Li and Noll, 1993, Germeraad et al., 1992) Df(2R)gsb^IIX (Matsuzaki and Saigo, 1996) Df(2R)gsb^zipper (Noordermeer et al., 1995) Df(2R)gspIIx62 (Wustmann et al., 1989) Df(2R)IIX62 (Tearle and Nusslein-Volhard, 1987, van den Heuvel et al., 1993, Martinez Arias, 1993, Duman-Scheel et al., 1997, McDonald and Doe, 1997, Klingler and Gergen, 1993, Ouellette et al., 1992, Warrior, 1994, Zhang et al., 1994, Young et al., 1993, Cote et al., 1987, Liu and Xue, 2012) unnamed (Bhat and Schedl, 1997)
Name Synonym
Secondary FlyBase IDs
	FBab0002180
References ( 43 )

Generate a list of	All references relating to this aberration ( 43 )

List References by type	Research paper ( 38 ) Review ( 1 ) Personal communication to FlyBase ( 1 ) FlyBase analysis ( 1 ) Stock list ( 1 ) Book ( 1 )
Recent research papers ( 1 )
	Liu and Xue, 2012, PLoS ONE 7(1): e30980 Functional Conservation of the Drosophila gooseberry Gene and Its Evolutionary Alleles. [FBrf0217339] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...