Aberration Dmel\Dp(1;f)1187

General Information
Symbol	Dmel\Dp(1;f)1187	Species	D. melanogaster
Name		FlyBase ID	FBab0003287
Feature type	free_duplication
Also Known As	Dp1187, Dp(1;f)8-23

Computed Breakpoints include	1B2;h26-h32
Duplicated segment	h26--1Rt
Sequence coordinates
Member of large scale dataset(s)
Recent Updates
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FB2013_03
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Nature of the Aberration
Cytological Order
Progenitor	In(1)sc⁸ (Karpen and Spradling, 1990, Tower et al., 1993)
Mutagen	X ray
Class of aberration (relative to progenitor)	free_duplication (Hawley et al., 1992)
Breakpoints	1B2;20 (Hawley et al., 1992)
Causes alleles
Carries alleles
Transposon Insertions
Formalized genetic data	ac << bk1 << bb << bk2
Genetic mapping information
Comments	45 insertions of P{ry⁺,lacZ} into Dp(1;f)1187 fell into the same 4.7kb hot spot of repeated DNA that demonstrates properties of heterochromatin. (Karpen and Spradling, 1992) 1300 kb (Karpen and Spradling, 1990, Cell 63: 97-107; Karpen and Spradling, unpublished)
Comments on Cytology
	Used in experiments that showed that efficient achiasmate homolog disjunction in female meiosis I requires 1000kb of overlap in the centric heterochromatin and is not affected by homologous euchromatin or overall size differences. Disjunction efficiency decreases linearly as heterochromatic overlap is reduced from 1000 to 430kb. Rescue experiments with nod transgenes showed that heterochromatin does not act solely to promote chromosome movement or spindle attachment. Centric heterochromatin seems to contain multiple pairing elements that act additively to initiate or maintain the proper alignment of achiasmate chromosomes in meiosis I. (Karpen et al., 1996) Ref: Karpen and Spradling, 1990, Cell 63: 97--107. (Hawley et al., 1992) Deletion results from breaks in the proximal and distal heterochromatin, exact location of the breakpoints is unknown. Coordinate 0 is the location of the existing In(1)sc⁸ breakpoint. ewg is present between coordinates -92 to -100, y between coordinates -20 to -25 and ac between coordinates -10 to -13. (Karpen and Spradling, 1990) Limits of break 1 from polytene analysis (FBrf0055832) Left limit of break 2 from inclusion of bb (citation unavailable) Right limit of break 2 from polytene analysis (FBrf0055832)
Sequence Crossreferences
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AF036950 L32746 L32747 U28039 U28050 Z32246 Z32477
Gene Deletion & Duplication Data
Genes Deleted / Disrupted
Complementation Data
Molecular Data
Genes NOT Deleted / Disrupted
Complementation Data
Molecular Data
Genes Duplicated
Complementation Data
Completely duplicated	ac (Karpen and Spradling, 1990) ewg (Karpen and Spradling, 1990) l(1)1Ac (Zhang and Spradling, 1993) l(1)1Ad (Zhang and Spradling, 1993) pch (Zhang and Spradling, 1993) y (Karpen and Spradling, 1990, Hawley et al., 1992)
Molecular Data
Genes NOT Duplicated
Complementation Data
	bb (Sawamura et al., 1993) sc (Hawley et al., 1992) su(f) (Sawamura et al., 1993) Zhr (Sawamura and Yamamoto, 1993, Sawamura et al., 1993)
Molecular Data
Related Comments

Phenotypic Data
In combination with other aberrations	The position effect variegation at the y locus caused by Dp(1;f)1187 is suppressed by Df(3R)Ace-HD1. (Cleard et al., 1997)
NOT in combination with other aberrations	The position effect variegation at the y locus caused by Dp(1;f)1187 is enhanced by Su(var)3-7^+t6.5. (Cleard et al., 1997) Variegated y phenotype scored by percentage of yellow bristles observed on the medial triple row at the anterior wing margin. (Henchoz et al., 1996) Variegated y phenotype, can be dominantly enhanced by mutations of E2f. (Seum et al., 1996) Transmission of the Dp(1;f)1187 minichromosome is sensitive to the dosage of nod⁺. Multiple regions of Dp(1;f)1187 interact with nod⁺ to promote normal chromosome transmission. Most nod⁺ interactions are observed with regions that are not essential for centromere function. (Murphy and Karpen, 1995) Assorts randomly with respect to sex chromosomes
Stocks ( 2 )
Bloomington	3940 Dp(1;f)1187, P{PZ}0801 P{PZ}8-23 y⁺; Df(1)sc⁸, y¹ sc⁸ w^a; ry⁵⁰⁶
Kyoto	107277 Dp(1;f)1187, P{PZ}0801 P{PZ}8-23 y⁺; In(1)sc⁸, Df(1)sc⁸, y¹ sc⁸ w^a; ry⁵⁰⁶
Notes on Origin
Discoverer	Krivshenko and Cooper, 1953.
	deletion of most of X euchromatin
Balancer / Genotype Variants of the Aberration
	Dp1187-8-23 Dp1187-0801 Dp1187-8001 Dp1187-8002 Dp1187-9901 Dp1187-Dp11 Dp1187-Dp12 Dp1187-Dp61 Dp1187-Dp62
Separable Components

Other Comments
	Length at mitotic metaphase (relative to length of 4th chromosome): 0.3 Frequency (%) of Dp/Y segregation among female progeny: 46.3 +- 1.19 (significant at 1% level of risk) (Portin and Rantanen, 1998.12.18) Acentric mini-chromosomes are lost at a moderate but elevated rate during male germline mitotic divisions and in female mitosis, but appear to be transmitted efficiently through pre-blastoderm mitoses and male meiosis. mit(1)15 can localise to the mini-chromosome. (Williams et al., 1998) The structure of heterochromatic DNA is altered as a general consequence of polyploid chromosome formation and the shortened molecules (restriction fragments that extend into the centric heterochromatin of Dp(1;f)1187) form as a consequence of heterochromatic underrepresentation. Alteration of heterochromatic DNA structure on Dp(1;f)1187 is not correlated with changes in the variegated expression of the y gene located on the minichromosome. (Glaser et al., 1997) Irradiation mutagenesis and pulse-field restriction mapping reveals significant amounts of substructure deep within centric heterochromatin. Heterochromatin in general is organised as alternating blocks of complex islands and satellite DNA, each hundreds of kilobases in length. Islands of DNA have been called Tahiti Moorea and Bora Bora. Tahiti has been cloned and sequences which revealed the presence of a Doc{}ry-OreR transposon. (Le et al., 1995) Centric heterochromatin contains islands of complex DNA (Tahiti, Moorea and Bora Bora) separated by blocks composed predominantly of highly repeated satellite DNA. Sequences necessary and sufficient for centromere activity during cell division reside in the island Bora Bora. (Murphy and Karpen, 1995) Satellite DNA carried on the chromosome is not involved in the hybrid lethality from the cross between D.melanogaster males and females of its sibling species. (Sawamura et al., 1995) While investigating the copy number of Dp(1;f)1187 sequences in the polyploid chromosome of ovarian nurse and follicle cells it was discovered that restriction fragments of genomic DNA spanning the euchromatic-heterochromatin junction have the unusual property of being selectively resistant to transfer out of agarose gels during Southern blotting, leading to systematic reductions in Dp(1;f)1187-specific hybridisation signals. Evidence is consistent with the hypothesis that pericentromeric sequences that are incorporated into heterochromatin in vivo retain some component of heterochromatic structure during DNA isolation which subsequently causes the reduced transfer efficiency observed in vitro. (Glaser and Spradling, 1994) Hybrids from the cross D.melanogaster C(1;Y)10/Dp(1;f)1187 males and D.sechellia females are viable. (Sawamura and Yamamoto, 1993) Progenitor chromosome for P{PZ} insertions P{PZ}3401, P{PZ}8-152, P{PZ}1803, P{PZ}0733, P{PZ}0453, P{PZ}2001, P{PZ}8801, P{PZ}655, P{PZ}885, P{PZ}0770, P{PZ}431 and P{PZ}0728. (Spradling, 1993) DNA from the euchromatic/heterochromatic junction region demonstrates a significant gradient of underreplication in larval salivary glands, lacked the stalled replication forks predicted by the underreplication model. Therefore underreplication is not the mechanism of heterochromatin underrepresentation. (Glaser et al., 1992) Induce low frequency of X and 4th chromosome nondisjunction. (Hawley et al., 1992) A strong long range position-effect on polytenization of euchromatin is seen. Reduced copy number probably contributes to gene dysfunction or alterations at the level of DNA metabolism cause some variegation position effects. Chromosome length is 1400kb, 1/25 size of a normal X chromosome. Euchromatin spans 320-400kb, the first 90kb of heterochromatin is distal and the remaining 1000kb is proximal. (Karpen and Spradling, 1990)
Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	1187 (Sawamura et al., 1994) Dp8-23 (Sullivan and Karpen, 2001) Dp1187 (Huertas et al., 2004, Cleveland et al., 2003, Rubin, 1998, Clarke, 1998, Wiens and Sorger, 1998, Wahlstrom et al., 1998, Sun et al., 1997, Williams et al., 1998, Karpen and Allshire, 1997, Allshire, 1997, Karpen et al., 1993, Glaser and Spradling, 1994, Donaldson and Karpen, 1997, Sun et al., 1996, Donaldson and Karpen, 1996, Cook et al., 1996, Le et al., 1995, Murphy and Karpen, 1995, Cook and Karpen, 1997, Hari and Karpen, 1997, Thomas and McKee, 2007) Dp(1;f)8-23 (Drapeau et al., 2006, Cleard et al., 1997, Drapeau et al., 2000, Drapeau et al., 2001) Dp(1;f)1187 (Thomas and McKee, 2007)
Name Synonym
Secondary FlyBase IDs

References ( 81 )

Generate a list of	All references relating to this aberration ( 81 )

List References by type	Research paper ( 43 ) Review ( 16 ) Personal communication to FlyBase ( 1 ) Abstract ( 19 ) FlyBase analysis ( 1 ) Book ( 1 )
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