|Name||Inversion (1) white-mottled||FlyBase ID||FBab0004257|
|Also Known As||wm4, whitem4|
|Computed Breakpoints include||3C2;h28|
|Member of large scale dataset(s)|
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|Nature of the Aberration|
|Class of aberration (relative to progenitor)|
|Formalized genetic data||l(1)2Ea << bk1 << w << CR << bk2|
|Genetic mapping information|
Breakpoint(s) molecularly mapped
Left (3C1-2) breakpoint at -24.5 kb in the restriction map of the w locus (0 point at site of the copia insertion in wa); right (20F) breakpoint near 5' end of a Type I mobile element (Tartof et al., 1984). Left breakpoint less than 3kb distal to the w-a copia insertion (Pirrotta et al., 1983).
|Comments on Cytology|
Distal inversion breakpoint is ~20kb from the 3' end of the w locus, the proximal inversion breakpoint is within centric heterochromatin distal to bb. Heterochromatic breakpoint is flanked by a Type I mobile element (FBrf0040503).
Proximal breakpoint is approximate. The distal breakpoint is to the left of w and the proximal breakpoint is to the left of bb.
|Gene Deletion & Duplication Data|
|Genes Deleted / Disrupted|
|Genes NOT Deleted / Disrupted|
|Genes NOT Duplicated|
|In combination with other aberrations|
The position effect variegation of the w gene seen in In(1)wm4 flies is partially suppressed by one copy of Df(2L)DS5 or Df(2L)DS6.
Variegation is not affected by Df(2R)pk-sple-51/+, Df(2R)nap2/+, Df(2R)sple-D2/+, Df(2R)sple-D1/+, Df(2R)pk30/+ or Df(2R)pk30/Df(2R)pk30/+.
Position effect variegation (PEV) at the w locus caused by In(1)wm4 is dominantly enhanced by Dp(2;2)Mdh and suppressed by Df(2R)en-A or Df(2R)en-SFX31.
The position effect variegation of w caused by In(1)wm4 is enhanced by Df(2L)cl-h1 and Df(2L)cl-h4. This phenotype is suppressed by Hel25E+t4.5; the level of variegation seen in In(1)wm4 ; Df(2L)cl-h4/Hel25E+t4.5 flies is the same as seen in In(1)wm4/+ flies.
|NOT in combination with other aberrations|
In(1)w[m4] silences w expression in most cells of the eye, leading to a variegated eye colour much lighter than normal.
Tn10\tetRey.3.5.T:Hsim\VP16-mediated expression of Hsap\HMGA1[MATH20.Tn10\tetO] in In(1)wm4 flies results in significant derepression of the w gene and a general loss of the variegating phenotype. Tetracycline treatment inhibits Hsap\HMGA1[MATH20.Tn10\tetO]-induced suppression of PEV. Tn10\tetRey.3.5.T:Hsim\VP16-mediated expression of Hsap\HMGA1[MATH11.Tn10\tetO] in In(1)wm4 flies results in no significant derepression of the w gene, no loss of the variegating phenotype.
Rpd315-1 enhances the variegation of w in In(1)wm4 flies and in 5-10% of the eyes patches of ommatidia show disorganisation characteristic of the rst mutation (enhancement of variegation allows the silencing to spread past the w locus to inactivate rst in a fraction of the cells).
Variegated eye phenotype is enhanced in the presence of mod(mdg4)ul, mod(mdg4)u2 and mod(mdg4)B2, eyes appear yellow with orange spots. In the presence of su(Hw)MC the phenotype is no longer enhanced and the eye phenotype returns to wild type. These results indicate that the variegating phenotype is caused by the su(Hw) protein.
z+ In(1)wm4 flies reared at 25oC have dark red/brown mottled eyes. z1 In(1)wm4 males and females have fewer red/brown patches on a lighter background, paired copies of In(1)wm4 are not repressed to z eye colour (lemon yellow). Repression of In(1)wm4 by z1 cannot be restored by Su(var)205. zv77h In(1)wm4 females and males have almost bleached white eye colour with a few scattered red facets, Su(var)205 only moderately suppresses PEV.
Carnitine compounds (L-Carnitine, L-Acetylcarnitine and L-Propionylcarnitine) show a significant suppression on w variegation. Butyrate gives a weaker suppression. In males the suppression effect of the compounds was seen at all concentrations, but in females the effect is weak and is only seen with high concentrations.
In(1)wm4 flies show position effect variegation at the w locus, at 23oC the phenotype varies from a sectored red and white eye to a red eye peppered with small flecks of red and brown pigmented ommatidia.
Eyes have a "sectored" phenotype - ommatidia are either fully pigmented or not pigmented at all.
Males and homozygous females are viable and fertile.
|Stocks ( 22 )|
|Notes on Origin|
|Balancer / Genotype Variants of the Aberration|
The presence of a variegating chromosome and a modifier chromosome in the same parental genome can alter the amount the amount of variegation formed in the progeny. The genomic imprinting is not determined by the parental origin of the variegating chromosome but is instead determined by the genetic background the variegating chromosome is subjected to during gametogenesis.
X ray-induced revertants of In(1)wm4 may or may not carry a segment of flanking heterochromatin: variegation is not controlled from immediately adjacent heterochromatic sequences at the euchromatin/ heterochromatin border but from a site more internal to the heterochromatin domain.
A strongly variegating line has been designated In(1)wm4h.
|Synonyms & Secondary IDs ( 13 )|
(Lerach et al., 2006, Sameny et al., 2011, Nakayama et al., 2007, Schwendemann et al., 2008, Wang et al., 2011, Schneiderman et al., 2009, Phalke et al., 2009, Zhu et al., 2008, Prabhakaran and Kelley, 2010, Vogel et al., 2009, Macdonald et al., 2010, Schneiderman et al., 2010, Di Stefano et al., 2011, Seong et al., 2011, Lloyd et al., 2003)
(Haley et al., 2005, Badugu et al., 2003, Malmanche and Clark, 2003, Schotta et al., 2002, Tulin et al., 2002, Granok et al., 2001, Reeves, 2001, Janssen et al., 2000, Shareef et al., 2001, Henikoff and Vermaak, 2000, Green and Piergentili, 2000, Buchner et al., 2000, Lefevre and Wilkins, 1966, Hart and Laemmli, 1998, Lefevre, 1968, Sass and Henikoff, 1998, Sinclair et al., 1998, Henikoff, 1990, Spradling and Karpen, 1990, Bezborodova et al., 1997, Larsson and Rasmuson-Lestander, 1997, The Moscow Regional Drosophila melanogaster Stock Center, Dubna, Tartof and Bremer, 1990, Cleard et al., 1997, Henikoff, 1996, Park and Yamamoto, 1995, Moehrle and Paro, 1994, Lloyd et al., 1997, Gdula et al., 1996, Shaffer et al., 1993, Tartof et al., 1984, Birchler et al., 1994, Sinclair et al., 1992, Weiler, 2001, Mason and Konev, 2001, Mason et al., 2002, Weiler, 2004, Bao et al., 2006, Lerach et al., 2006, McHugh et al., 2004, Maines et al., 2007, Rudolph et al., 2007, Eggert et al., 2004, Weiler, 2007, Fagegaltier et al., 2009, Zhu et al., 2008, Vogel et al., 2009, Schneiderman et al., 2010, Nakayama et al., 2012, Seong et al., 2011, Lloyd et al., 2003, Smolik, 2009, Stephens et al., 2006)
Inversion (1) white-mottled
white mottled 4
|Secondary FlyBase IDs|
|References ( 159 )|
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|Recent research papers ( 5 )|
|Recent reviews (0)|
|All reviews listed in FlyBase were published before 2011|