FlyBase Aberration Report: Dmel\Df(2L)C144

General Information

Symbol

Df(2L)C144

Species

D. melanogaster

Name

FlyBase ID

FBab0022184

Feature type

chromosomal_deletion

Computed Breakpoints include

22F3-22F4;23C3-23C5

Features within 22F4--23C3 (Estimated cytology)

Genomic Maps

JBrowse

Sequence coordinates

2L:2,517,598..2,551,864 (Df(2L)C144:bk1)

(Cook et al., 2010.1.8)

2L:2,955,279..2,961,962 (Df(2L)C144:bk2)

(Cook et al., 2010.1.8)

Member of large scale dataset(s)

Nature of Aberration

Cytological Order

Progenitor

Mutagen

Class of aberration (relative to wild type)

chromosomal_deletion

(FlyBase, 2007)

Class of aberration (relative to progenitor)

chromosomal_deletion

(Ghabrial et al., 1998, Sekelsky et al., 1995, Littleton et al., 1993)

Breakpoints

23A1-23A2;23C

(Littleton et al., 1993)

23A1-23A2;23C3-23C5

(Ghabrial et al., 1998, Sekelsky et al., 1995)

Causes alleles

Rbp9^C144

(Cook et al., 2010.1.8)

Carries alleles

Transposon Insertions

Formalized genetic data

oaf << bk1 << Drp1 << l(2)23AB2 << bk2 << okr

Genetic mapping information

Comments

This deletion removes a single ribosomal protein-coding gene (though other genes are also removed).

(Marygold et al., 2007)

Comments on Cytology

The left breakpoint lies within CG3528 or CG3515 or in the region between them, and lies in the range 2L:2517598..2551864 (R5) (predicted cytology: 22F4). The right breakpoint lies within Rbp9, and lies in the range 2L:2955279..2961962 (R5) (predicted cytology: 23C3).

(Cook et al., 2010.1.8)

PCR amplification of the Chd1 genomic region from flies carrying Df(2L)C144 over a large Chd1 deletion shows that the right limit of breakpoint 2 for Df(2L)C144 lies to the left of Chd1.

(McDaniel, 2006.10.11)

Left limit of break 1 from non-inclusion of oaf (FBrf0076536) Right limit of break 1 from inclusion of Drp1 (FBrf0076536) Limits of break 2 from polytene analysis (FBrf0080380)

Sequence Crossreferences

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

Gene Deletion and Duplication Data

Genes Deleted / Disrupted

Complementation Data

Completely deleted / disrupted

aph-1

(DiAntonio et al., 1993)

daw

(Parker et al., 2006)

Drp1

(Ly, 2005.3.2, Littleton and Bellen, 1994)

Duox

(Hurd et al., 2015, Stefancsik, 2002.5.22, DiAntonio et al., 1993)

Hrs

(DiAntonio et al., 1993)

huc2L-16

(Silva et al., 2006)

l(2)23Ab

(Kahsai and Cook, 2018)

l(2)23AB2

(DiAntonio et al., 1993)

l(2)23AB4

(DiAntonio et al., 1993)

l(2)23AB7

(Littleton and Bellen, 1994, DiAntonio et al., 1993)

l(2)k16525

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

lilli

(Pepple et al., 2007, Su et al., 2001, Wittwer et al., 2001, Spradling et al., 1999, BDGP Project Members, 1994-1999)

nrd

(Kahsai and Cook, 2018)

Pgk

(Wang et al., 2004, DiAntonio et al., 1993)

RpS21

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Syt1

(Kahsai et al., 2016, Stefancsik, 2002.5.22, Spradling et al., 1999, BDGP Project Members, 1994-1999, Littleton and Bellen, 1994, DiAntonio et al., 1993, Littleton et al., 1993)

Partially deleted / disrupted

Molecular Data

Completely deleted

Drp1

(McDaniel, 2006.10.11)

l(2)23AB2

(McDaniel, 2006.10.11)

RpS21

(Marygold et al., 2007)

Partially deleted

Rbp9

(Cook et al., 2010.1.8)

Genes NOT Deleted / Disrupted

Complementation Data

Molecular Data

Chd1

(McDaniel, 2006.10.11)

Genes Duplicated

Complementation Data

Completely duplicated

Partially duplicated

Molecular Data

Completely duplicated

Partially duplicated

Genes NOT Duplicated

Complementation Data

Molecular Data

Affected Genes Inferred by Location (79)

Related Comments

Phenotypic Data

In combination with other aberrations

Lethal in combination with Df(2L)BSC156.

(Kahsai et al., 2016)

Fails to complement T(2;3)DTD46.4.

(Su et al., 2001)

NOT in combination with other aberrations

Homozygous lethal.

(Hurd et al., 2015)

Flies heterozygous for the deletion show a Minute bristle phenotype.

(Marygold et al., 2007)

The Df(2L)C144 chromosome does not act as a dominant suppressor of telomeric silencing (assayed using the effect of the chromosome on the eye colour phenotype of flies carrying "P{w^var}KR3-2", a stable "brown-red" variant of the P{3'WP-2,w^var}2Lt insertion).

(Mason et al., 2004)

Heterozygosity for Df(2L)C144 results in 2.3% X chromosome nondisjunction and 0.0% fourth chromosome nondisjunction in In(1)FM7/X ; sv^spa-pol females.

(Harris et al., 2003)

Wild-type clones made in the ovaries of a mutant female, are significantly larger than clones of heterozygous mutant cells. This size difference is greater in clones initiated at 48 hours after egg laying (AEL) than 2 hours AEL.

(Extavour and Garcia-Bellido, 2001)

No second site non-complementing phenotype with zip^Ebr and zip^mhc-c6.1.

(Halsell and Kiehart, 1998)

Stocks (3)

Bloomington

Df(2L)C144, dpp^d-ho ed¹/In(2LR)Gla, wg^Gla-1 PPO1^Bc Egfr^E1

Kyoto

103265

Df(2L)C144, dpp^d-ho ed¹ / wg^Gla-1 PPO1^Bc Egfr^E1

108889

Df(2L)C144, dpp^d-ho ed¹/In(2LR)Gla, wg^Gla-1 PPO1^Bc Egfr^E1

Notes on Origin