FlyBase Aberration Report: Dmel\Df(2L)N22-14

General Information

Symbol

Df(2L)N22-14

Species

D. melanogaster

Name

FlyBase ID

FBab0022196

Feature type

chromosomal_deletion

Computed Breakpoints include

29C1-29C2;30C8-30C9;30D1-30D2;31A1-31A2

Features within 29C1--30C9

Genomic Maps

JBrowse

Sequence coordinates

2L:8,399,212..8,402,391 [-] (Df(2L)N22-14:bk1)

2L:8,399,332..8,401,129 (Df(2L)N22-14:bk1)

2L:9,792,596..9,806,483 (Df(2L)N22-14:bk2)

(Yamamoto et al., 2009.2.25)

Member of large scale dataset(s)

Nature of Aberration

Cytological Order

2Lt - 29C1 | 30C9 - 30D1 | 31A1 - 30D2 | 31A2 - 2Rt

(Ashburner, 1992.5.27)

Progenitor

P{en-lacZ(wXba)}9

(Neuman-Silberberg and Schupbach, 1993)

Mutagen

gamma ray

(Neuman-Silberberg and Schupbach, 1993)

Class of aberration (relative to wild type)

chromosomal_deletion

Class of aberration (relative to progenitor)

Breakpoints

29C1--2;[]

(Neuman-Silberberg and Schupbach, 1993)

29C1-29C2;30C8-30C9;30D1-30D2;31A1-31A2

(Ashburner, 1992.5.27)

29C1-29C2;30C8-30C9

(Collier and Gubb, 1997)

Causes alleles

Carries alleles

Transposon Insertions

Formalized genetic data

bk1 << fy << grk << bk2

Genetic mapping information

Comments

This deletion removes multiple ribosomal protein-coding genes (in addition to other genes).

(Marygold et al., 2007)

Breakpoint 1 was mapped to a BamHI-HindIII restriction fragment. The position of the restriction fragment on the reference sequence was inferred by a FlyBase curator.

(Collier and Gubb, 1997)

Breakpoint 1 was mapped to an EcoRI-EcoRI restriction fragment. The position of the restriction fragment on the reference sequence was inferred by a FlyBase curator.

(Neuman-Silberberg and Schupbach, 1993)

Comments on Cytology

The left Df(2L)N22-14 breakpoint lies in CG13386 or CG31898 or in the region between them, and lies in the range 2L:8399332..8401129 (R5) (predicted cytology: 29C1).

(Yamamoto et al., 2009.2.25)

The right Df(2L)N22-14 breakpoint lies within CG13123 or nAcRα-30D or the region between them, and lies in the range 2L:9792596..9806483 (R5) (predicted cytology: 30D1).

(Yamamoto et al., 2009.2.25)

Limits of break 1 from polytene analysis (FBrf0065509) Limits of break 2 from polytene analysis (FBrf0089969) Limits of break 3 from polytene analysis (FBrf0089969) Limits of break 4 from polytene analysis (FBrf0089969)

Sequence Crossreferences

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

Gene Deletion and Duplication Data

Genes Deleted / Disrupted

Complementation Data

Completely deleted / disrupted

Partially deleted / disrupted

Molecular Data

Completely deleted

mRpL51

(Marygold et al., 2007)

RpS28-like

(Marygold et al., 2007)

Partially deleted

Genes NOT Deleted / Disrupted

Complementation Data

Dad1

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

l(2)gl

(Mason et al., 2004)

l(2)k07118b

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

l(2)k14816

(BDGP Project Members, 1994-1999)

RpS13

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

snRNA:U6atac

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Molecular Data

Genes Duplicated

Complementation Data

Completely duplicated

Partially duplicated

Molecular Data

Completely duplicated

Partially duplicated

Genes NOT Duplicated

Complementation Data

Molecular Data

Affected Genes Inferred by Location (0)

If no genes are listed here, it may be because the affected region is very large. The JBrowse insert above may show an error for the same reason, and other FlyBase tools such as CytoSearch may also fail for large regions. You can contact FlyBase for more help.
In these cases, there will be no "Export to Hitlist" button to the left.

Related Comments

Phenotypic Data

In combination with other aberrations

Lethal in combination with T(Y;2)fy⁴.

(Collier et al., 2000, Collier and Gubb, 1997)

NOT in combination with other aberrations

Flies heterozygous for the deletion do not show a Minute bristle phenotype.

(Marygold et al., 2007)

Little if any macrophage migration occurs in homozygous embryos, with most macrophages remaining in the anterior region, clustered around the foregut, at stage 13-15.

(Paladi and Tepass, 2004)

Heterozygosity for Df(2L)N22-14 results in 1.5% X chromosome nondisjunction and 0.0% fourth chromosome nondisjunction in In(1)FM7/X ; sv^spa-pol females.

(Harris et al., 2003)

Heterozygotes show a quantitative effect on wing shape in intervein region C compared to wild type.

(Palsson and Gibson, 2000)

Does not cause unconditional lethality in hybrid females when heterozygous with D.simulans chromosome.

(Coyne et al., 1998)

Stocks (2)

2892

107489

Notes on Origin

Discoverer

The stock contains a second-site deletion uncovering l(2)gl.

(Roegiers et al., 2009)

Balancer / Genotype Variants of the Aberration

Separable Components

Other Comments

The Df(2L)N22-14 chromosome acts as a dominant weak suppressor of telomeric silencing (assayed using the effect of the chromosome on the eye colour phenotype of flies carrying "P{w^var}KR3-2", a stable "brown-red" variant of the P{3'WP-2,w^var}2Lt insertion), but this is assumed to be a false positive result (the suppressor may not be within the bounds of the deficient region) because the 2L TAS array on the chromosome is reduced (as assayed by in situ hybridization) and it has previously been shown (FBrf0137248, FBrf0158986) that partial or complete deficiency of the 2L TAS array on the homologue suppresses silencing of brown-red variants of P{3'WP-2,w^var}2Lt.

(Mason et al., 2004)

Deletes in a proximal direction from stated breakpoint. Cytology of second break not reported.

(Neuman-Silberberg and Schupbach, 1993)

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

Df(2)N22-14

(Anderson et al., 2006)

Df(2L)M22-14

(Quezada-Díaz et al., 2010)

Df(2L)N22

(Coyne et al., 1998)

Df(2L)N22 - 14

(Babu and Ramachandra, 2007)

Df(2L)N22-14

(Pan et al., 2022, Bala Tannan et al., 2018, Clough et al., 2014, Cook, 2014.9.25, Bolkan et al., 2012, Chen and Ganetzky, 2012, Fernandes and Rao, 2011, Watson et al., 2010, Roegiers et al., 2009, Yamamoto et al., 2009.2.25, Casso et al., 2008, Lin et al., 2008, Levine et al., 2007, Fry and Saweikis, 2006, Silva et al., 2006, Paladi and Tepass, 2004)

Df(2L)N22¹⁴

(Ashton et al., 2001)

N22-14

(Mao et al., 2006)

Name Synonyms

Roote 2-42

(Rawls and Wolff, 2003)

Secondary FlyBase IDs

References (48)

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