Aberration Dmel\Df(2L)N22-14

General Information
Symbol	Dmel\Df(2L)N22-14	Species	D. melanogaster
Name		FlyBase ID	FBab0022196
Feature type	chromosomal_deletion

Computed Breakpoints include	29C1-29C2;30C8-30C9;30D1-30D2;31A1-31A2
Deleted segment	29C1--30C9
Map ( GBrowse )	Untitled Document
Sequence coordinates	2L:8,399,212..8,402,391 [-] (Df(2L)N22-14:bk1) (Collier and Gubb, 1997, Neuman-Silberberg and Schupbach, 1993) 2L:8,399,332..8,401,129 (Df(2L)N22-14:bk1) (Yamamoto et al., 2009.2.25) 2L:9,792,596..9,806,483 (Df(2L)N22-14:bk2) (Yamamoto et al., 2009.2.25)
Member of large scale dataset(s)
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Aberration
Cytological Order	2Lt - 29C1 \| 30C9 - 30D1 \| 31A1 - 30D2 \| 31A2 - 2Rt (Ashburner, 1992.5.27)
Progenitor	P{en-lacZ(wXba)}9 (Neuman-Silberberg and Schupbach, 1993)
Mutagen	gamma ray (Neuman-Silberberg and Schupbach, 1993)
Class of aberration (relative to progenitor)
Breakpoints	29C1--2;[] (Neuman-Silberberg and Schupbach, 1993) 29C1-29C2;30C8-30C9 (Collier and Gubb, 1997) 29C1-29C2;30C8-30C9;30D1-30D2;31A1-31A2 (Ashburner, 1992.5.27)
Causes alleles
Carries alleles
Transposon Insertions
Formalized genetic data	bk1 << fy << grk << bk2
Genetic mapping information
Comments
Comments on Cytology
	The left Df(2L)N22-14 breakpoint lies in CG13386 or CG31898 or in the region between them, and lies in the range 2L:8399332..8401129 (R5) (predicted cytology: 29C1). (Yamamoto et al., 2009.2.25) The right Df(2L)N22-14 breakpoint lies within CG13123 or nAcRα-30D or the region between them, and lies in the range 2L:9792596..9806483 (R5) (predicted cytology: 30D1). (Yamamoto et al., 2009.2.25) Limits of break 1 from polytene analysis (FBrf0065509) Limits of break 2 from polytene analysis (FBrf0089969) Limits of break 3 from polytene analysis (FBrf0089969) Limits of break 4 from polytene analysis (FBrf0089969)
Sequence Crossreferences
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name
Gene Deletion & Duplication Data
Genes Deleted / Disrupted
Complementation Data
Completely deleted / disrupted	Akap200 (Jackson and Berg, 2002) Aldh (Fry and Saweikis, 2006) C1GalTA (Lin et al., 2008) d (Mao et al., 2006) emb (Spradling et al., 1999, Collier et al., 2000, BDGP Project Members, 1994-1999) fy (Ashburner, 1992.5.27, Collier and Gubb, 1997) grk (Neuman-Silberberg and Schupbach, 1993) huc29DE (Silva et al., 2006) ifm(2)RS3 (Babu and Ramachandra, 2007) lmgA (BDGP Project Members, 1994-1999) mus201 (Sekelsky et al., 2000) nAcRα-30D (Watson et al., 2010) Orct (Spradling et al., 1999) raw (Byars et al., 1999) zf30C (Cook, 2002.3.7)
Molecular Data
Genes NOT Deleted / Disrupted
Complementation Data
	l(2)gl (Mason et al., 2004) l(2)k07118b (Spradling et al., 1999, BDGP Project Members, 1994-1999) l(2)k12914 (Spradling et al., 1999, BDGP Project Members, 1994-1999) l(2)k14816 (BDGP Project Members, 1994-1999) RpS13 (Spradling et al., 1999, BDGP Project Members, 1994-1999) snRNA:U6atac:29B (Spradling et al., 1999, BDGP Project Members, 1994-1999)
Molecular Data
Genes Duplicated
Complementation Data
Molecular Data
Genes NOT Duplicated
Complementation Data
Molecular Data
Related Comments

Phenotypic Data
In combination with other aberrations	Lethal in combination with T(Y;2)fy⁴. (Collier and Gubb, 1997, Collier et al., 2000)
NOT in combination with other aberrations	Little if any macrophage migration occurs in homozygous embryos, with most macrophages remaining in the anterior region, clustered around the foregut, at stage 13-15. (Paladi and Tepass, 2004) Heterozygosity for Df(2L)N22-14 results in 1.5% X chromosome nondisjunction and 0.0% fourth chromosome nondisjunction in In(1)FM7/X ; sv^spa-pol females. (Harris et al., 2003) Heterozygotes show a quantitative effect on wing shape in intervein region C compared to wild type. (Palsson and Gibson, 2000) Does not cause unconditional lethality in hybrid females when heterozygous with D.simulans chromosome. (Coyne et al., 1998)
Stocks ( 2 )
Bloomington	2892 Df(2L)N22-14/CyO
Kyoto	107489 Df(2L)N22-14/CyO
Notes on Origin
Discoverer
	The stock contains a second-site deletion uncovering l(2)gl. (Roegiers et al., 2009)
Balancer / Genotype Variants of the Aberration

Separable Components

Other Comments
	The Df(2L)N22-14 chromosome acts as a dominant weak suppressor of telomeric silencing (assayed using the effect of the chromosome on the eye colour phenotype of flies carrying "P{w^var}KR3-2", a stable "brown-red" variant of the P{3'WP-2,w^var}2Lt insertion), but this is assumed to be a false positive result (the suppressor may not be within the bounds of the deficient region) because the 2L TAS array on the chromosome is reduced (as assayed by in situ hybridization) and it has previously been shown (FBrf0137248, FBrf0158986) that partial or complete deficiency of the 2L TAS array on the homologue suppresses silencing of brown-red variants of P{3'WP-2,w^var}2Lt. (Mason et al., 2004) Deletes in a proximal direction from stated breakpoint. Cytology of second break not reported. (Neuman-Silberberg and Schupbach, 1993)
Synonyms & Secondary IDs ( 8 )
Reported As
Symbol Synonym	Df(2)N22-14 (Anderson et al., 2006) Df(2L)M22-14 (Quezada-Díaz et al., 2010) Df(2L)N22 (Coyne et al., 1998) Df(2L)N22-14 (Fry and Saweikis, 2006, Bolkan et al., 2012, Casso et al., 2008, Yamamoto et al., 2009.2.25, Roegiers et al., 2009, Lin et al., 2008, Silva et al., 2006, Paladi and Tepass, 2004, Watson et al., 2010, Levine et al., 2007, Chen and Ganetzky, 2012) Df(2L)N22 - 14 (Babu and Ramachandra, 2007) Df(2L)N22¹⁴ (Ashton et al., 2001) N22-14 (Mao et al., 2006)
Name Synonym	Roote 2-42 (Rawls and Wolff, 2003)
Secondary FlyBase IDs

References ( 40 )

Generate a list of	All references relating to this aberration ( 40 )

List References by type	Research paper ( 35 ) Personal communication to FlyBase ( 4 ) Abstract ( 1 )
Recent research papers ( 2 )
	Bolkan et al., 2012, J. Neurosci. 32(46): 16181--16192 beta-Secretase Cleavage of the Fly Amyloid Precursor Protein Is Required for Glial Survival. [FBrf0219983] Chen and Ganetzky, 2012, J. Cell Biol. 196(4): 529--543 A neuropeptide signaling pathway regulates synaptic growth in Drosophila. [FBrf0217500] Show all research papers ...