Aberration Dmel\Df(1)NP5

General Information
Symbol	Dmel\Df(1)NP5	Species	D. melanogaster
Name		FlyBase ID	FBab0026716
Feature type	inversion_derived_deficiency_plus_aneuploid
Also Known As	NP5

Computed Breakpoints include
Sequence coordinates
Member of large scale dataset(s)
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Aberration
Cytological Order
Progenitor	In(1)366.2 (Mitchell et al., 1996, Mitchell, 1997.2.17) In(1)P363 (Mitchell et al., 1996, Mitchell, 1997.2.17)
Mutagen	recombination (Mitchell et al., 1996, Mitchell, 1997.2.17)
Class of aberration (relative to progenitor)
Breakpoints
Causes alleles
Carries alleles
Transposon Insertions
Formalized genetic data	bk1 << NetA << rut << bk4
Genetic mapping information
Comments
Comments on Cytology
	The recombinant chromosome is deficient for 12E9-12E11;12F2-5 and has the inversion breakpoints 12F2-12F5;17C2. Another inversion, roughly In(1)2;10, is also present. (Mitchell, 1997.2.17)
Sequence Crossreferences
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name
Gene Deletion & Duplication Data
Genes Deleted / Disrupted
Complementation Data
Completely deleted / disrupted	NetA (Keleman and Dickson, 2001, Kolesnikov and Beckendorf, 2005, Mitchell et al., 1996, Mitchell, 1997.2.17, Sano et al., 2005, Al-Anzi and Wyman, 2009) NetB (Keleman and Dickson, 2001, Kolesnikov and Beckendorf, 2005, Mitchell et al., 1996, Mitchell, 1997.2.17, Sano et al., 2005, Al-Anzi and Wyman, 2009) rut (Mitchell et al., 1996, Mitchell, 1997.2.17)
Molecular Data
Genes NOT Deleted / Disrupted
Complementation Data
	mud (Guan et al., 2000, Mitchell, 1997.2.17) yl (Mitchell et al., 1996, Mitchell, 1997.2.17)
Molecular Data
Genes Duplicated
Complementation Data
Molecular Data
Genes NOT Duplicated
Complementation Data
Molecular Data
Related Comments

Phenotypic Data
In combination with other aberrations
NOT in combination with other aberrations	Df(1)NP5 mutants exhibit fragmented commissures and gaps in longitudinal connectives. (Al-Anzi and Wyman, 2009) 90% of Df(1)NP5/Y embryos show defects in axon guidance in the Bolwig's nerve. Df(1)NP5 embryos show defects in the commissures of the central nervous system; 2% of anterior commissures are absent, 4% of anterior commissures are thin, 8% of posterior commissures are absent and 15% of posterior commissures are thin. 10% of segments fail to separate the anterior and posterior commissures correctly. (Andrews et al., 2008) Df(1)NP5 embryos exhibit salivary gland guidance defects. In wild-type embryos the salivary glands lie parallel to the CNS midline, while 23% of Df(1)NP5 embryos have glands that curve away laterally and 9% have glands that curve medially towards the midline. This phenotype occurs as the glands migrate along the visceral mesoderm, as the invagination of the salivary glands is normal. Gut constriction is not affected in these mutants. The commissures of the ventral nerve cord are abnormally thin or completely absent in Df(1)NP5 embryos. (Kolesnikov and Beckendorf, 2005) Embryos heterozygous for Df(1)NP5, removing one copy of NetA and NetB exhibit normal commissures, however the longitudinal tracts, when visualized with BP102 antibody, are thinner between segments and thicker within segments. When fascicles are visualized with Fas2, embryos homozygous or heterozygous for Df(1)NP5 reveal irregularities and interruptions in the longitudinal Fas2 bundles. The heterozygote Df(1)NP5 phenotype is not enhanced in embryos also heterozygous for sli^unspecified, robo^unspecified, or scb^unspecified. In contrast, the frequency of midline guidance errors is increased in sli^unspecified/+ or robo^unspecified/+, but not scb^unspecified embryos also heterozygous for Df(1)NP5. (Stevens and Jacobs, 2002) No abnormalities are seen in SNa and SNc projections. Lateral migration of peripheral and exit glia appears normal. (Keleman and Dickson, 2001) In mutant embryos, the there are defects in the posterior commissure and there are breaks in the longitudinal connectives. (Bashaw and Goodman, 1999) Homozygous lethal, lethality is rescued by Dp(1;f)LJ9 and Dp(1;f)LJ4. Hemizygous embryos display a range of phenotypes in the CNS, most notably a thinning or absence of the commissures in the ventral nerve cord. This defect can be rescued by restoring midline expression of either NetA or NetB gene. (Mitchell, 1997.2.17) Homozygous lethal. Lethality can be rescued by Dp(1;f)LJ9. Embryo exhibits CNS phenotype: commissures are much thinner and sometimes completely absent, occasional breaks in the longitudinal tracts. Commissural phenotype is rescued by expression of P{slit-NetA} or P{slit-NetB}. There are no segmentation defects, no gross changes in the fate or pattern of midline cells or CNS neurons, no increase in cell death. Embryos exhibit occasional errors in the projection pattern of the motor nerves, including lack if innervation of muscles 6 and 7. Projection of sensory axons into the CNS is almost completely normal. (Mitchell et al., 1996)
Stocks ( 2 )
Bloomington	2179 Df(1)NP5, hdp-b^P363/FM7c, P{act-lacZ.B}GD1 P{act-lacZ.B}GD2
Kyoto	106687 Df(1)NP5, hdp-b^P363/FM7c, P{act-lacZ.B}GD1 P{act-lacZ.B}GD2
Notes on Origin
Discoverer

Balancer / Genotype Variants of the Aberration

Separable Components

Other Comments

Synonyms & Secondary IDs ( 8 )
Reported As
Symbol Synonym	Df(1)NP3 (Mitchell et al., 1996) Df(1)NP5 (Orgogozo et al., 2004, Keleman and Dickson, 2001, Guan et al., 2000, Hiramoto et al., 2000, Mitchell, 1997.2.17, Mitchell et al., 1996, Forsthoefel et al., 2003, Sano et al., 2005, Andrews et al., 2008, Song et al., 2011) Df(1)NP-5 (Al-Anzi and Wyman, 2009) Df-NP5 (Garbe et al., 2007) In(1)366.2^LP363^R In(1)N366.2^LP363^R (Mitchell, 1997.2.17) NetA,B^NP5 (Andrews et al., 2008) NP5 (Kolesnikov and Beckendorf, 2005, Bashaw and Goodman, 1999)
Name Synonym
Secondary FlyBase IDs

References ( 17 )
Research paper	Song et al., 2011, J. Genet. Genomics 38(1): 1--11 TRIM-9 functions in the UNC-6/UNC-40 pathway to regulate ventral guidance. [FBrf0213082] Al-Anzi and Wyman, 2009, Neural Dev. 4: 31 The Drosophila immunoglobulin gene turtle encodes guidance molecules involved in axon pathfinding. [FBrf0209104] Andrews et al., 2008, Development 135(23): 3839--3848 Dscam guides embryonic axons by Netrin-dependent and -independent functions. [FBrf0206236] Garbe et al., 2007, Development 134(24): 4325--4334 Cytoplasmic domain requirements for Frazzled-mediated attractive axon turning at the Drosophila midline. [FBrf0200520] Forsthoefel et al., 2005, Development 132(8): 1983--1994 The Abelson tyrosine kinase, the Trio GEF and Enabled interact with the Netrin receptor Frazzled in Drosophila. [FBrf0183931] Kolesnikov and Beckendorf, 2005, Dev. Biol. 284(1): 102--111 NETRIN and SLIT guide salivary gland migration. [FBrf0187388] Sano et al., 2005, J. Cell Biol. 171(4): 675--683 Control of lateral migration and germ cell elimination by the Drosophila melanogaster lipid phosphate phosphatases Wunen and Wunen 2. [FBrf0190943] Orgogozo et al., 2004, Mech. Dev. 121(5): 427--436 Slit-Robo signalling prevents sensory cells from crossing the midline in Drosophila. [FBrf0179366] Stevens and Jacobs, 2002, J. Neurosci. 22(11): 4448--4455 Integrins regulate responsiveness to slit repellent signals. [FBrf0149077] Keleman and Dickson, 2001, Neuron 32(4): 605--617 Short- and long-range repulsion by the Drosophila Unc5 Netrin receptor. [FBrf0141688] Guan et al., 2000, Proc. Natl. Acad. Sci. U.S.A. 97(14): 8122--8127 Mushroom body defect, a gene involved in the control of neuroblast proliferation in Drosophila, encodes a coiled-coil protein. [FBrf0128481] Bashaw and Goodman, 1999, Cell 97(7): 917--926 Chimeric axon guidance receptors: the cytoplasmic domains of slit and netrin receptors specify attraction versus repulsion. [FBrf0108593] Mitchell et al., 1996, Neuron 17(2): 203--215 Genetic analysis of Netrin genes in Drosophila: Netrins guide CNS commissural axons and peripheral motor axons. [FBrf0089749]
Personal communication to FlyBase	Bloomington Drosophila Stock Center, 2004.9.24, Formal aberration symbols in FlyBase. Formal aberration symbols in FlyBase. [FBrf0179867] Mitchell, 1997.2.17, In(1)N366.2<up>L</up>P363<up>R</up> and Df(1)NP5. In(1)N366.2^LP363^R and Df(1)NP5. [FBrf0093819]
Abstract	Forsthoefel et al., 2003, A. Dros. Res. Conf. 44: 703A abelson, trio, and enabled interact genetically with frazzled. [FBrf0154556]
Letter	Hiramoto et al., 2000, Nature 406(6798): 886--889 The Drosophila Netrin receptor Frazzled guides axons by controlling Netrin distribution. [FBrf0129858]