A set of ~50 targeted deficiencies created by exploiting hybrid element insertion (HEI) and resolution; designed to fill gaps in deletion coverage.
65D4-65D5;65E4-65E6
The 3L:6942786 release 6 coordinate of the left breakpoint is an estimate. It corresponds to the insertion site of P{lacW}l(3)L4060L4060. As FBrf0175003 explains, deletions generated by P transposase in the presence of trans-heterozygous P elements are expected to extend from the end of one P insertion to the vicinity of the other P insertion. The absence of miniwhite from the Df(3L)BSC27 chromosome suggests that the deletion extends from the left end of the P{PZ}corn04202 insertion to the vicinity of P{lacW}l(3)L4060L4060.
The 3L:7156003 release 6 coordinate of the right breakpoint is an estimate. It corresponds to the insertion site of P{PZ}corn04202. As FBrf0175003 explains, deletions generated by P transposase in the presence of trans-heterozygous P elements are expected to extend from the end of one P insertion to the vicinity of the other P insertion. The absence of miniwhite from the Df(3L)BSC27 chromosome suggests that the deletion extends from the left end of the P{PZ}corn04202 insertion to the vicinity of P{lacW}l(3)L4060L4060.
The Df(3L)BSC27 chromosome does not act as a dominant suppressor of telomeric silencing (assayed using the effect of the chromosome on the eye colour phenotype of flies carrying "P{wvar}KR3-2", a stable "brown-red" variant of the P{3'WP-2,wvar}2Lt insertion).
The miniwhite marker from P{lacW}l(3)L4060L4060 was deleted or disrupted.
