[48A3-48A3];[48D5-48D5];
A set of isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Initial core set of 209 isogenic deletions provides ~60% euchromatic genome coverage.
48A3;48D5
Df(2R)BSC879/Df(2R)ED2247 embryos do not accumulate the tracheal chitin filament and the trachea remain uninflated. A normal pattern of migration and tracheal cell specification is observed, but branches completely lack luminal and apical-cuticular chitin. General apicobasal polarity and adhesion are not affected. The dorsal trunk has a cystic appearance, with many dilations and constrictions, and the presence of apical expansions, usually at joints between branches; there are also abnormal cell shapes in the dorsal trunk.
Df(2R)BSC879/Df(2R)ED2247 embryos show a fragmented and granular head skeleton and an inflated and misshapen larval shape. The tracheal envelope and epicuticle is detached from the epithelial cells, while the space between these structures and the epithelial cells is devoid of material. The envelope and the epicuticle of the integument is decreased in thickness and the procuticle is amorph.
Heterozygotes show hypersensitivity to halothane and enflurane compared to controls.
Confirmed by 3-step PCR confirmation as shown in http://www.drosdel.org.uk/del_confirm.php Confirmed by lack of complementation to a known lethal mutation predicted to fall between the progenitor insertions.
Limits of break 1 computationally determined from location of progenitor P insertion on genome sequence between P{lacW}Tapδk17005&P{lacW}k05103 and P{lacW}l(2)k13403k13403&P{lacW}Egmk14708 Limits of break 2 computationally determined from location of progenitor P insertion on genome sequence between P{lacW}l(2)k06612k06612 and P{lacW}jebk05644