FB2025_01 , released February 20, 2025
Aberration: Dmel\Df(3L)ED225
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General Information
Symbol
Df(3L)ED225
Species
D. melanogaster
Name
FlyBase ID
FBab0035337
Feature type
chromosomal_deletion
Also Known As
ED225
Computed Breakpoints include

[75C1-75C1];[75D4-75D4];

Features within 75C1--75D4Features within 75C1--75C2 (Observed cytology)
Genomic Maps
Sequence coordinates
3L:18,186,145..18,186,145 (Df(3L)ED225:bk1)
(DrosDel Project, 2007.6.25)
3L:18,621,337..18,621,337 (Df(3L)ED225:bk2)
(DrosDel Project, 2007.6.25)
Member of large scale dataset(s)
Dfs_DrosDel_set1

A set of isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Initial core set of 209 isogenic deletions provides ~60% euchromatic genome coverage.

(Ryder et al., 2007)
Nature of Aberration
Cytological Order
Progenitor
P{RS3r}CB-5465-3
(Ryder, 2003.6.17)
P{RS5r}5-HA-1189
(Ryder, 2003.6.17)
Mutagen
FLPase
(Ryder, 2003.6.17)
Class of aberration (relative to wild type)
chromosomal_deletion
(FlyBase, 2007)
Class of aberration (relative to progenitor)
chromosomal_deletion
(Ryder, 2003.6.17)
Breakpoints

75C1;75D4

(FlyBase Curators, 2008)
Causes alleles
hidED225
(Tan et al., 2011)
Carries alleles
Transposon Insertions
P{3'.RS5+3.3'}ED225
(Ryder, 2003.6.17)
Formalized genetic data
Genetic mapping information
Comments

Df(3L)ED225 deletes all but 1507bp of W upstream regulatory sequence, leaving the coding region of W intact, and extends proximally to delete grim, rpr, skl and further proximal material.

(Mohseni et al., 2009)
Comments on Cytology

This deficiency removes the 75C1, 75C1′ (a new small band between 75C1 and 75C2) and 75C2 bands, replacing them with a fine unknown band “X” visible between the bands 75B9-10 and 75C4; the band “X” most probably comprises the material of the 75B11-13 fine bands and the distal part of the 75C1 band.

(Andreyenkova et al., 2009)

Confirmed by 3-step PCR confirmation as shown in http://www.drosdel.org.uk/del_confirm.php Confirmed by lack of complementation to a known lethal mutation predicted to fall between the progenitor insertions.

(Ryder and Roote, 2005.11.4)

Limits computationally determined from location of progenitor P insertion on genome sequence between P{PZ}W05014 and P{lacW}l(3)j14E7j14E7

Sequence Crossreferences
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
Gene Deletion and Duplication Data
Genes Deleted / Disrupted
Complementation Data
Completely deleted / disrupted
grim
(Mohseni et al., 2009)
hid
(Mohseni et al., 2009)
rpr
(Mohseni et al., 2009)
skl
(Mohseni et al., 2009)
Partially deleted / disrupted
hid
(Tan et al., 2011)
Molecular Data
Completely deleted
grim
(Mohseni et al., 2009)
rpr
(Mohseni et al., 2009)
skl
(Mohseni et al., 2009)
Partially deleted
hid
(Mohseni et al., 2009)
Genes NOT Deleted / Disrupted
Complementation Data
 
jaft
(Collins and Cohen, 2005)
Molecular Data
 
Genes Duplicated
Complementation Data
Completely duplicated
Partially duplicated
Molecular Data
Completely duplicated
Partially duplicated
Genes NOT Duplicated
Complementation Data
 
Molecular Data
 
Affected Genes Inferred by Location (42)
Phenotypic Data
In combination with other aberrations

Df(3L)X25/Df(3L)ED225 results in lethality.

(Lee et al., 2013)

Df(3L)XR38/Df(3L)ED225 animals have a defect in the programmed cell death of vCrz neurons: 15.3 +/- 0.5 neurons are present at 7 hours after puparium formation (these neurons are no longer present in wild-type animals at this stage).

(Lee et al., 2013)

Df(3L)grim-A6C/Df(3L)ED225 animals have a defect in the programmed cell death of vCrz neurons: 16 +/- 0 neurons are present at 7 hours after puparium formation (APF) (these neurons are no longer present in wild-type animals at this stage) and 16 +/- 0 neurons are present at 16 hours APF. 8.8 +/- 1.5 surviving EW3-sib cells are seen in these animals (these cells die during embryogenesis in wild type).

(Lee et al., 2013)

Inferred to overlap with: Df(3L)MM2.

(Tan et al., 2011)

Ventral neuroblast-clusters of Df(3L)MM2/Df(3L)ED225 mutant larvae display supernumerary cells compared with wild-type. Df(3L)MM2/Df(3L)ED225 is semi-lethal. The flies that emerge live for only one or two days.

(Tan et al., 2011)

Heterozygotes enhance the position effect variegation of w seen in the In(1)wm4h chromosome.

(Zhao et al., 2008)
NOT in combination with other aberrations

Df(3L)ED225 mutant embryos show a significantly decreased number of cells in all ventral nerve cord segments (T1 to T3 and A1 to A9 to A9-10), associated with increased proportion of apoptotic cells (casp3-positive cells), and show a significant increase in the mitotic index of neuroblasts, but not of neuroblast daughter cells, only in T3 segment, as compared to controls.

(Monedero Cobeta et al., 2017)

Df(3L)ED225/Df(3L)ED225 mutant embryos display increased proliferation of thoracic neuroblast daughter cells cells at stage 14, and abdominal neuroblasts at stages 14 and 15, but no significant difference in proliferation of thoracic neuroblasts at stages 12, 14 or 15, thoracic neuroblast daughter cells at stage 12 or 15, abdominal neuroblasts at stage 12, or abdominal neuroblast daughter cells at stage 12, 14 or 15, as compared with controls. These mutants show no significant change in number of EL neurons, as compared with controls.

(Bivik et al., 2016)

Heterozygous animals have a defect in the programmed cell death of vCrz neurons: 9.6 +/- 1.9 neurons are present at 7 hours after puparium formation (these neurons are no longer present in wild-type animals at this stage).

(Lee et al., 2013)

In contrast to controls, no chromosomal breaks in the 75C1-2 region are found in chromosomes bearing the deficiency.

(Andreyenkova et al., 2009)
Stocks (2)
Notes on Origin
Discoverer
 
Balancer / Genotype Variants of the Aberration
 
Separable Components
 
Other Comments
 
Synonyms and Secondary IDs (5)
References (27)