Aberration Dmel\Df(3L)ED4543

General Information
Symbol	Dmel\Df(3L)ED4543	Species	D. melanogaster
Name		FlyBase ID	FBab0035791
Feature type	chromosomal_deletion

Computed Breakpoints include	[70C6-70C6];[70F4-70F4];
Deleted segment	70C6--70F4
Map ( GBrowse )	Untitled Document
Sequence coordinates	3L:13,928,325..13,928,325 (Df(3L)ED4543:bk1) (DrosDel Project, 2007.6.25) 3L:14,751,140..14,751,140 (Df(3L)ED4543:bk2) (DrosDel Project, 2007.6.25)
Member of large scale dataset(s)	Dfs_DrosDel_set1 A set of isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Initial core set of 209 isogenic deletions provides ~60% euchromatic genome coverage. (Ryder et al., 2007)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Aberration
Cytological Order
Progenitor	P{RS3r}CB-6268-3 (Ryder, 2003.6.17) P{RS5r}5-SZ-3479 (Ryder, 2003.6.17)
Mutagen	FLPase (Ryder, 2003.6.17)
Class of aberration (relative to progenitor)	chromosomal_deletion (Ryder, 2003.6.17)
Breakpoints	70C6;70F4 (FlyBase Curators, 2008)
Causes alleles
Carries alleles
Transposon Insertions	P{3'.RS5+3.3'}ED4543 (Ryder, 2003.6.17)
Formalized genetic data
Genetic mapping information
Comments
Comments on Cytology
	Confirmed by 3-step PCR confirmation as shown in http://www.drosdel.org.uk/del_confirm.php Confirmed by lack of complementation to a known lethal mutation predicted to fall between the progenitor insertions. (Ryder and Roote, 2005.11.4) Limits of break 1 computationally determined from location of progenitor P insertion on genome sequence between P{lacW}l(3)L5212^L5212&P{PZ}l(3)05871⁰⁵⁸⁷¹ and P{EP}EP3561^EP3561 Limits of break 2 computationally determined from location of progenitor P insertion on genome sequence between P{PZ}Mpcp⁰⁰⁵⁶⁴ and P{PZ}l(3)rO220^rO220
Sequence Crossreferences
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name
Gene Deletion & Duplication Data
Genes Deleted / Disrupted
Complementation Data
Completely deleted / disrupted	ptip (Fang et al., 2009)
Molecular Data
Genes NOT Deleted / Disrupted
Complementation Data
Molecular Data
Genes Duplicated
Complementation Data
Molecular Data
Genes NOT Duplicated
Complementation Data
Molecular Data
Related Comments

Phenotypic Data
In combination with other aberrations	Df(3L)ED4502/Df(3L)ED4543 flies are viable. (Ryder et al., 2007)
NOT in combination with other aberrations
Stocks ( 2 )
Bloomington	8073 w¹¹¹⁸; Df(3L)ED4543, P{3'.RS5+3.3'}ED4543/TM6C, cu¹ Sb¹
Kyoto	150434 w¹¹¹⁸; Df(3L)ED4543 / TM6C, Sb¹
Notes on Origin
Discoverer

Balancer / Genotype Variants of the Aberration

Separable Components

Other Comments

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	Df(3L)ED4543 (Ryder et al., 2007, FlyBase Curators, 2008, Fang et al., 2009, Edwards and Mackay, 2009, McAnally and Yampolsky, 2010, Popkova et al., 2012, Weber et al., 2012) Df(ptip)² (Fang et al., 2009) ED4543 (Shao et al., 2010)
Name Synonym
Secondary FlyBase IDs

References ( 13 )
Research paper	Popkova et al., 2012, PLoS Genet. 8(12): e1003159 Polycomb controls gliogenesis by regulating the transient expression of the gcm/glide fate determinant. [FBrf0220515] Weber et al., 2012, Genetics 191(1): 145--162 Novel regulators of planar cell polarity: a genetic analysis in Drosophila. [FBrf0218210] McAnally and Yampolsky, 2010, Genome Biol. Evol. 2010: 44--52 Widespread transcriptional autosomal dosage compensation in Drosophila correlates with gene expression level. [FBrf0210383] Shao et al., 2010, PLoS ONE 5(4): e9938 A modifier screen for Bazooka/PAR-3 interacting genes in the Drosophila embryo epithelium. [FBrf0210449] Edwards and Mackay, 2009, Genetics 182(3): 889--897 Quantitative Trait Loci for Aggressive Behavior in Drosophila melanogaster. [FBrf0208371] Fang et al., 2009, Development 136(11): 1929--1938 Drosophila ptip is essential for anterior/posterior patterning in development and interacts with the PcG and trxG pathways. [FBrf0207992] Ryder et al., 2007, Genetics 177(1): 615--629 The DrosDel deletion collection: a Drosophila genomewide chromosomal deficiency resource. [FBrf0202170]
Personal communication to FlyBase	DrosDel Project, 2007.6.25, DrosDel deletion data mapped to Release 5.1 (updated 2007-06-25). DrosDel deletion data mapped to Release 5.1 (updated 2007-06-25). [FBrf0205060] Ryder and Roote, 2005.11.4, Update from DrosDel Database - Release 4 mappings - November 2005. Update from DrosDel Database - Release 4 mappings - November 2005. [FBrf0188738] Ryder, 2003.6.17, Drosdel deletions. Drosdel deletions. [FBrf0161091]
FlyBase analysis	Millburn et al., 2012.8.1, Lines in aberration datasets (files on ftp site). Lines in aberration datasets (files on ftp site). [FBrf0219195] FlyBase Curators, 2008, Release 5 estimated cytology of aberrations with sequence-defined breakpoints. Release 5 estimated cytology of aberrations with sequence-defined breakpoints. [FBrf0206255] FlyBase, 2007, En masse symbol-based assigment of Aberration Class with respect to wild type. En masse symbol-based assigment of Aberration Class with respect to wild type. [FBrf0191808]