Aberration Dmel\Df(3R)ED5516

General Information
Symbol	Dmel\Df(3R)ED5516	Species	D. melanogaster
Name		FlyBase ID	FBab0036749
Feature type	chromosomal_deletion

Computed Breakpoints include	[86D8-86D8];[86E13-86E13];
Deleted segment	86D8--86E13
Map ( GBrowse )	Untitled Document
Sequence coordinates	3R:7,059,892..7,059,892 (Df(3R)ED5516:bk1) (DrosDel Project, 2007.6.25) 3R:7,445,622..7,445,622 (Df(3R)ED5516:bk2) (DrosDel Project, 2007.6.25)
Member of large scale dataset(s)	Dfs_DrosDel_set1 A set of isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Initial core set of 209 isogenic deletions provides ~60% euchromatic genome coverage. (Ryder et al., 2007)
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Aberration
Cytological Order
Progenitor	P{RS3r}UM-8176-3 (Ryder and Roote, 2004.11.10) P{RS5r}5-SZ-3031 (Ryder and Roote, 2004.11.10)
Mutagen	FLPase (Ryder and Roote, 2004.11.10)
Class of aberration (relative to progenitor)	chromosomal_deletion (Ryder, 2003.6.17, Ryder and Roote, 2004.11.10)
Breakpoints	86D8;86E13 (FlyBase Curators, 2008)
Causes alleles
Carries alleles
Transposon Insertions	P{3'.RS5+3.3'}ED5516 (Ryder and Roote, 2004.11.10)
Formalized genetic data
Genetic mapping information
Comments
Comments on Cytology
	Confirmed by 3-step PCR confirmation as shown in http://www.drosdel.org.uk/del_confirm.php Confirmed by lack of complementation to a known lethal mutation predicted to fall between the progenitor insertions. (Ryder and Roote, 2005.11.4) Confirmed by PCR (using the 3-step process described on http://www.drosdel.org.uk/del_confirm.php). (Ryder and Roote, 2004.11.10) Limits of break 1 computationally determined from location of progenitor P insertion on genome sequence between P{EP}EP3340^EP3340 and P{PZ}tho¹&P{PZ}l(3)04629⁰⁵²⁷⁵ Limits of break 2 computationally determined from location of progenitor P insertion on genome sequence between P{PZ}tho¹&P{PZ}l(3)04629⁰⁵²⁷⁵ and P{EP}Lk6^EP886
Sequence Crossreferences
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name
Gene Deletion & Duplication Data
Genes Deleted / Disrupted
Complementation Data
Completely deleted / disrupted	pros (Ryder and Roote, 2004.11.10)
Molecular Data
Genes NOT Deleted / Disrupted
Complementation Data
	cu (Roote, 2004.6.24) RpL3 (Ryder and Roote, 2004.11.10) RpS25 (Roote, 2004.6.24)
Molecular Data
Genes Duplicated
Complementation Data
Molecular Data
Genes NOT Duplicated
Complementation Data
Molecular Data
Related Comments

Phenotypic Data
In combination with other aberrations	Viable in combination with Df(3R)MS32. (Cook, 2006.10.1)
NOT in combination with other aberrations	Does not show a Minute phenotype: analysis aimed at identification of RpS25. (Roote, 2004.6.24)
Stocks ( 2 )
Bloomington	8968 w¹¹¹⁸; Df(3R)ED5516, P{3'.RS5+3.3'}ED5516/TM6C, cu¹ Sb¹
Kyoto	150042 w¹¹¹⁸; Df(3R)ED5516/TM6C, Sb¹
Notes on Origin
Discoverer
	Potential deficiency; could be generated as the recombinant product between the reduced derivatives of P{RS3}UM-8176-3 and P{RS5}5-SZ-3031. (Ryder, 2003.6.17)
Balancer / Genotype Variants of the Aberration

Separable Components

Other Comments

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Df(3R)ED5516 (Cook, 2006.10.1, FlyBase Curators, 2008, Weber et al., 2012)
Name Synonym
Secondary FlyBase IDs

References ( 11 )
Research paper	Weber et al., 2012, Genetics 191(1): 145--162 Novel regulators of planar cell polarity: a genetic analysis in Drosophila. [FBrf0218210] Ryder et al., 2007, Genetics 177(1): 615--629 The DrosDel deletion collection: a Drosophila genomewide chromosomal deficiency resource. [FBrf0202170]
Personal communication to FlyBase	DrosDel Project, 2007.6.25, DrosDel deletion data mapped to Release 5.1 (updated 2007-06-25). DrosDel deletion data mapped to Release 5.1 (updated 2007-06-25). [FBrf0205060] Cook, 2006.10.1, M(3)S32 and M(3)86D correspond to RpS25. M(3)S32 and M(3)86D correspond to RpS25. [FBrf0199175] Ryder and Roote, 2005.11.4, Update from DrosDel Database - Release 4 mappings - November 2005. Update from DrosDel Database - Release 4 mappings - November 2005. [FBrf0188738] Roote, 2004.6.24, ED genetic data. ED genetic data. [FBrf0178881] Ryder and Roote, 2004.11.10, DrosDel deletions confirmed by PCR and genetic tests. DrosDel deletions confirmed by PCR and genetic tests. [FBrf0179888] Ryder, 2003.6.17, Drosdel deletions. Drosdel deletions. [FBrf0161091]
FlyBase analysis	Millburn et al., 2012.8.1, Lines in aberration datasets (files on ftp site). Lines in aberration datasets (files on ftp site). [FBrf0219195] FlyBase Curators, 2008, Release 5 estimated cytology of aberrations with sequence-defined breakpoints. Release 5 estimated cytology of aberrations with sequence-defined breakpoints. [FBrf0206255] FlyBase, 2007, En masse symbol-based assigment of Aberration Class with respect to wild type. En masse symbol-based assigment of Aberration Class with respect to wild type. [FBrf0191808]