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General Information
Symbol
Df(1)Exel6235
Species
D. melanogaster
Name
FlyBase ID
FBab0037801
Feature type
Computed Breakpoints include

[5A2-5A2];[5A6-5A6];

Deleted Segment
Sequence coordinates
X:5,516,611..5,516,611 (Df(1)Exel6235:bk1)
X:5,593,966..5,593,966 (Df(1)Exel6235:bk2)
Member of large scale dataset(s)
Dfs_Exelixis_set1

A set of isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Initial set of 519 isogenic deletions provides 56% genome coverage.

The current Exelixis collection at the Bloomington Stock Center differs from the original described in FBrf0175003 : a significant number were shown not to carry a deletion and have been removed from the collection; a number of stocks have been lost; a number of additional deletions are included that were generated after publication.

Nature of Aberration
Cytological Order
Progenitor
Class of aberration (relative to wild type)
Class of aberration (relative to progenitor)
Causes alleles
Carries alleles
Transposon Insertions
Formalized genetic data
Genetic mapping information
Comments

Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.

69932 bp deletion which removes all of Vsx2 and CG12730 and 32.5 kb of intergenic DNA. The deletion ends 334bp before the start of the dVsx1 5'UTR.

Comments on Cytology

Limits computationally determined from location of progenitor P insertion on genome sequence between P{EP}CG3249EP1400 and P{EP}Tre1EP496

Sequence Crossreferences
DNA sequence
Protein sequence
Gene Deletion and Duplication Data
Genes Deleted / Disrupted
Complementation Data
Completely deleted / disrupted
Partially deleted / disrupted
Molecular Data
Partially deleted
Genes NOT Deleted / Disrupted
Complementation Data
Molecular Data
Genes Duplicated
Complementation Data
Completely duplicated
Partially duplicated
Molecular Data
Completely duplicated
Partially duplicated
Genes NOT Duplicated
Complementation Data
 
Molecular Data
 
Phenotypic Data
In combination with other aberrations
NOT in combination with other aberrations

The outer optic anlagen and brain are reduced in size in Df(1)Exel6235 mutant second instar larvae. Mutant larval brains show defects in proliferation as measured by the number of phospho-histone 3 (PH3) positive cells compared to controls; the outer optic anlagen shows a 5.07-fold decrease in PH3-positive cells, the inner optic anlagen shows a 2.51-fold decrease in PH3-positive cells and the medial brain shows a 2.82-fold decrease in PH3-positive cells

Mutants show lethality at all postembryonic stages. Mutants show developmental defects, with the time taken to reach the molt to second instar from hatching being increased relative to controls.

Mutant pharate adults have significantly smaller optic lobes than control animals. The optic lobes are also disorganised; the incoming photoreceptor axons have marked projection errors in both the lamina and deeper layers of the optic lobe, and in the most severe cases the projections terminate deep in the brain.

The third larval instar outer optic anlage (OOA) is dramatically reduced in size in mutant animals compared to controls, and is not visible at all in some mutant brains. The inner optic anlage appears grossly normal in these animals.

The OOA appears normal in shape and size in mutant stage 16 embryos, however defects are seen in early second instar larvae, where the OOA is hypoplastic and disorganised, with the arms of the OOA failing to form the U-shaped structure seen in controls.

Stocks (1)
Notes on Origin
Discoverer
 
Balancer / Genotype Variants of the Aberration
 
Separable Components
 
Other Comments
 
Synonyms and Secondary IDs (3)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (10)