A set of isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Initial set of 519 isogenic deletions provides 56% genome coverage.
The current Exelixis collection at the Bloomington Stock Center differs from the original described in FBrf0175003 : a significant number were shown not to carry a deletion and have been removed from the collection; a number of stocks have been lost; a number of additional deletions are included that were generated after publication.
Df(2R)KT40/Df(2R)Exel6070 transheterozygotes show excessive or disorganized basal stalk cells, mis-shaped follicle cells, a multilayered follicular epithelium and/or aberrant nurse cell number, as compared to controls; the multilayering is virtually absent under starvation conditions. Under starvation, these transheterozygotes show a significant increase in the mitotic index of the follicular epithelium, as compared to controls.
The aforementioned defects are strongly rescued by the adulthood-only expression of either hhHMS00492 or Rab23JF02859 under the control of Scer\GAL4bab1-Pgal4-2 (and Gal80[ts] for temporal control of expression).