A set of isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Initial set of 519 isogenic deletions provides 56% genome coverage.
The current Exelixis collection at the Bloomington Stock Center differs from the original described in FBrf0175003 : a significant number were shown not to carry a deletion and have been removed from the collection; a number of stocks have been lost; a number of additional deletions are included that were generated after publication.
Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.
Df(2R)Exel7157/Df(2R)Exel7158 results in semi-lethality. Those animals that do survive to adulthood have no obvious morphological abnormalities and their viability is not at all compromised for at least two weeks in the absence of lithium treatment. However, the mutant adults are susceptible to 50mM LiCl; their general activity is severely impaired within one day of treatment, followed by death within the next few days (median time to lethality is 2.3 days).