A set of isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Initial set of 519 isogenic deletions provides 56% genome coverage.
The current Exelixis collection at the Bloomington Stock Center differs from the original described in FBrf0175003 : a significant number were shown not to carry a deletion and have been removed from the collection; a number of stocks have been lost; a number of additional deletions are included that were generated after publication.
Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.
Df(3L)Exel6128/Df(3L)BSC559 flies or Df(3L)Exel6128/Df(3L)BSC560 flies, derived from heterozygous parents, are viable, fertile and have no discernable phenotypes despite the fact these deficiencies overlap by 55 kb of genomic DNA. However, when these flies are inbred for several generations, their progeny often have thin bristles and etched abdominal tergites. These phenotypes are not rescued by either of two paternally inherited duplications (Ts(YSt;3Lt)ST1 or Dp(3;Y)L131-D3), indicating that the phenotypes are not caused by deleting the 55 kb genomic region, but by a maternal-effect mutation somewhere else in the genome.