A set of isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Initial set of 519 isogenic deletions provides 56% genome coverage.
The current Exelixis collection at the Bloomington Stock Center differs from the original described in FBrf0175003 : a significant number were shown not to carry a deletion and have been removed from the collection; a number of stocks have been lost; a number of additional deletions are included that were generated after publication.
Neuromuscular junction (NMJ) synapses appear relatively normal ultrastructurally in homozygous embryos, except that pre- and postsynaptic densities are incompletely apposed. The number of synaptic vesicles, dense core vesicles, active zone diameter and postsynaptic density diameter are all statistically indistinguishable from wild type. NMJ size and the number of active zones/NMJ are significantly reduced in the homozygous embryos compared to wild type. The amplitude of the evoked excitatory junction current (EJC) is reduced in homozygous embryonic NMJs compared to wild type, while the frequency of spontaneous EJCs is unaffected. There is no change in the amplitude of glutamate-gated currents at the NMJ in these embryos.