A set of isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Initial set of 519 isogenic deletions provides 56% genome coverage.
The current Exelixis collection at the Bloomington Stock Center differs from the original described in FBrf0175003 : a significant number were shown not to carry a deletion and have been removed from the collection; a number of stocks have been lost; a number of additional deletions are included that were generated after publication.
The wings from Df(3R)Exel6194/Df(3R)irk2B mutant flies show wing vein defects, including incomplete or branched posterior cross veins, incomplete L5 vein, bifurcations of L3 and L4 veins and wing bristle transformations. 39% of flies have held-out wings from hinge defects. Rare Df(3R)Exel6194/Df(3R)irk2B mutant wings are notched or smaller than wild type. The penetrance and severity of wing phenotypes are dependent both on sex and the temperature during development; the most severe phenotypes are seen in males raised at low temperatures, with 38% of male flies having wing defects at 25[o]C.