[96C4-96C4];[96C5-96C5];
A set of isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Initial set of 519 isogenic deletions provides 56% genome coverage.
The current Exelixis collection at the Bloomington Stock Center differs from the original described in FBrf0175003 : a significant number were shown not to carry a deletion and have been removed from the collection; a number of stocks have been lost; a number of additional deletions are included that were generated after publication.
96C4;96C5
Df(3R)Exel9056 does not obviously delete any genes by microarray analysis.
Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.
Homozygotes die at late pupal stages.
30% of Df(3R)Exel9056 embryos succumb to wounding, whereas most wild type embryos survive after receiving a puncture wound.
Whereas wild type embryos seal their wounds within 60 minutes, 26% of Df(3R)Exel9056 embryos fail to close their wounds within 3 hours and the remaining mutants take an average of 110 minutes to seal their wounds.
There are no gross defects in cellular protrusions at wound margins of Df(3R)Exel9056 embryos. However, whereas wild type controls show a pronounced actin cable around the wound edge halfway through the healing process, Df(3R)Exel9056 embryos show either a discontinuous or a significantly less intense actin cable around the wound.
