[67B1-67B1];[67B5-67B5];
A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
67B1;67B5
Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.
Presence of P+PBac{XP5.RB3}BSC113 was verified using the PCR methods and primers described in FBrf0175003 with the substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the Supplementary Methods.
The cytological breakpoints of Df(3L)BSC113 predicted from the Release 3 genome coordinates of progenitor insertions P{XP}d07693 and PBac{RB}aaye04497 are 67A8;67B4.