[65E9-65E9];[65F5-65F5];
A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
65E9-65F1;65F2-65F5
65E9;65F5
Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.
Inferred to overlap with: Df(3L)BSC33.
Lethal in combination with Df(3L)BSC33. Inferred to overlap with: Df(3L)BSC33.
Presence of P+PBac{XP5.RB3}BSC117 was verified using the PCR methods and primers described in FBrf0175003 with the substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the Supplementary Methods.
The cytological breakpoints of Df(3L)BSC117 predicted from the Release 3 genomic coordinates of PBac{RB}e04444 and P{XP}CG8600d02813 are 65F2;65F5; however our polytene chromosome squashes showed the breakpoints 65E9-F1;65F2-5 (the 65E8,9 doublet band and 65F5 are present; the 65F1,2 doublet band is absent).