[35B8-35B8];[35C1-35C1];
A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
35B8;35C1
Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.
The presence of P+PBac{XP5.WH5}BSC299 was verified using the PCR methods and primers described in FBrf0175003.
Exelixis, Inc. determined the insertion site of PBac{WH}f03562 to be at Release 3 genomic coordinate 14988104 on chromosome arm 2L. The Gene Disruption project determined the insertion site of PBac{WH}f03562 to be at Release 3 genomic coordinate 14988091 on arm 2L. This corresponds to 35B8 on the Release 5 genome map. The predicted position of P{XP}d09327 on the Release 5 map is 35C1. Consequently, the cytological breakpoints of Df(2L)BSC299 are predicted to be 35B8;35C1.