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General Information
Symbol
Df(2L)BSC217
Species
D. melanogaster
Name
FlyBase ID
FBab0045090
Feature type
Computed Breakpoints include

[24D8-24D8];[24F1-24F1];

Deleted Segment
Sequence coordinates
2L:4,197,800..4,197,800 (Df(2L)BSC217:bk1)
2L:4,404,013..4,404,013 (Df(2L)BSC217:bk2)
Member of large scale dataset(s)
Dfs_BSC_set2

A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.

Nature of Aberration
Cytological Order
Class of aberration (relative to wild type)
Class of aberration (relative to progenitor)
Breakpoints
Causes alleles
Carries alleles
Formalized genetic data
Genetic mapping information
Comments

Breakpoint based on release 3 sequence coordinate from Thibault et al., 2004, Supplementary Table 2 (FBrf0174227) or 3 (FBrf0174228), converted to release 5 coordinate.

Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.

This deletion removes a single ribosomal protein-coding gene (though other genes are also removed).

Comments on Cytology

The cytological breakpoints of Df(2L)BSC217 was determined by PCR to be at Release 3 genomic coordinate 4395974 on chromosome arm 2L, a site predicted to be within 24F1 on both the Release 3 and Release 4 genome maps. The predicted position of P{XP}d09957 on the Release 4 map is 24D8. Consequently, the predicted cytological breakpoints of Df(2L)BSC217 are 24D8;24F1.

Sequence Crossreferences
DNA sequence
Protein sequence
Gene Deletion and Duplication Data
Genes Deleted / Disrupted
Complementation Data
Partially deleted / disrupted
Molecular Data
Completely deleted
Partially deleted
Genes NOT Deleted / Disrupted
Complementation Data
 
Molecular Data
 
Genes Duplicated
Complementation Data
Completely duplicated
Partially duplicated
Molecular Data
Completely duplicated
Partially duplicated
Genes NOT Duplicated
Complementation Data
 
Molecular Data
 
Phenotypic Data
In combination with other aberrations
NOT in combination with other aberrations

Heterozygous Df(2L)BSC217 mutant females exhibit a dilated cardiomyopathy phenotype, including an increased end-systolic dimension (EDD) and a decreased fractional shortening (FS) compared to controls. Bristles appear short and thin compared to wild type (Minute phenotype).

Flies heterozygous for the deletion show a Minute bristle phenotype.

Df(2L)BSC217 heterozygosity gives rise to a Minute bristle phenotype from deletion of RpL40.

Stocks (1)
Notes on Origin
Discoverer
 
Balancer / Genotype Variants of the Aberration
 
Separable Components
 
Other Comments
 

The presence of P+PBac{XP5.WH5}BSC217 was verified using the PCR methods and primers described in FBrf0175003.

Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (11)