[24D8-24D8];[24F1-24F1];
A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
24D8;24F1
Breakpoint based on release 3 sequence coordinate from Thibault et al., 2004, Supplementary Table 2 (FBrf0174227) or 3 (FBrf0174228), converted to release 5 coordinate.
Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.
This deletion removes a single ribosomal protein-coding gene (though other genes are also removed).
Heterozygous Df(2L)BSC217 mutant females exhibit a dilated cardiomyopathy phenotype, including an increased end-systolic dimension (EDD) and a decreased fractional shortening (FS) compared to controls. Bristles appear short and thin compared to wild type (Minute phenotype).
Flies heterozygous for the deletion show a Minute bristle phenotype.
Df(2L)BSC217 heterozygosity gives rise to a Minute bristle phenotype from deletion of RpL40.
The presence of P+PBac{XP5.WH5}BSC217 was verified using the PCR methods and primers described in FBrf0175003.
The cytological breakpoints of Df(2L)BSC217 was determined by PCR to be at Release 3 genomic coordinate 4395974 on chromosome arm 2L, a site predicted to be within 24F1 on both the Release 3 and Release 4 genome maps. The predicted position of P{XP}d09957 on the Release 4 map is 24D8. Consequently, the predicted cytological breakpoints of Df(2L)BSC217 are 24D8;24F1.