Aberration Dmel\Df(2L)BSC323

General Information
Symbol	Dmel\Df(2L)BSC323	Species	D. melanogaster
Name		FlyBase ID	FBab0045130
Feature type	chromosomal_deletion

Computed Breakpoints include	[33A5-33A5];[33C1-33C1];
Deleted segment	33A5--33C1
Map ( GBrowse )	Untitled Document
Sequence coordinates	2L:11,870,517..11,870,517 (Df(2L)BSC323:bk1) (Bloomington Drosophila Stock Center, 2008.8.7) 2L:12,055,450..12,055,450 (Df(2L)BSC323:bk2) (Bloomington Drosophila Stock Center, 2008.8.7)
Member of large scale dataset(s)	Dfs_BSC_set2 A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped. (Cook et al., 2012)
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Aberration
Cytological Order
Progenitor	P{XP}d10374 (Christensen et al., 2007.10.29) PBac{WH}Pde1c^f02409 (Christensen et al., 2007.10.29)
Mutagen	FLPase (Christensen et al., 2007.10.29)
Class of aberration (relative to progenitor)	chromosomal_deletion (Christensen et al., 2007.10.29)
Breakpoints	33A5;33C1 (FlyBase Curators, 2008)
Causes alleles
Carries alleles
Transposon Insertions	P+PBac{XP5.WH5}BSC323 (Christensen et al., 2007.10.29)
Formalized genetic data
Genetic mapping information
Comments
Comments on Cytology
	Exelixis, Inc. determined the insertion site of P{XP}d10374 to be at Release 3 genomic coordinate 12044040 on chromosome arm 2L. The Gene Disruption project determined the insertion site of P{XP}d10374 to be at Release 3 genomic coordinate 12044441 on arm 2L. This corresponds to 33C1 on both the Release 3 and 5 genome maps. The predicted position of PBac{WH}CG31757^f02409 on the Release 5 map is 33A5. Consequently, the cytological breakpoints of Df(2L)BSC323 are predicted to be 33A5;33C1. (Christensen et al., 2007.10.29)
Sequence Crossreferences
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name
Gene Deletion & Duplication Data
Genes Deleted / Disrupted
Complementation Data
Molecular Data
Genes NOT Deleted / Disrupted
Complementation Data
Molecular Data
Genes Duplicated
Complementation Data
Molecular Data
Genes NOT Duplicated
Complementation Data
Molecular Data
Related Comments

Phenotypic Data
In combination with other aberrations	Inferred to overlap with: Df(2L)Exel6030. (Christensen et al., 2007.10.29) Inferred to overlap with: Df(2L)Exel6031. (Christensen et al., 2007.10.29)
NOT in combination with other aberrations	Df(2L)BSC323 is homozygous lethal. (Bergwitz et al., 2012)
Stocks ( 1 )
Bloomington	24348 w¹¹¹⁸; Df(2L)BSC323/CyO
Notes on Origin
Discoverer

Balancer / Genotype Variants of the Aberration

Separable Components

Other Comments
	The presence of P+PBac{XP5.WH5}BSC323 was verified using the PCR methods and primers described in FBrf0175003. (Christensen et al., 2007.10.29)
Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Df(2L)BSC323 (Christensen et al., 2007.10.29, Bergwitz et al., 2012, FlyBase, 2007, FlyBase Curators, 2008, Cook et al., 2012) Df(2L)FDD-0292951 (Christensen et al., 2007.10.29)
Name Synonym
Secondary FlyBase IDs

References ( 8 )
Research paper	Bergwitz et al., 2012, PLoS ONE 7(2): e31730 Roles of major facilitator superfamily transporters in phosphate response in Drosophila. [FBrf0217562] Cook et al., 2012, Genome Biol. 13(3): R21 The generation of chromosomal deletions to provide extensive coverage and subdivision of the Drosophila melanogaster genome. [FBrf0219066]
Supplementary material	Cook et al., 2012, Genome Biol. 13(3): Additional file 1. Breakpoints and characterization of BSC deletions. [FBrf0219069]
Personal communication to FlyBase	Bloomington Drosophila Stock Center, 2008.8.7, Release 5 sequence coordinates of BSC deficiencies. Release 5 sequence coordinates of BSC deficiencies. [FBrf0205560] Christensen et al., 2007.10.29, Isolation and characterization of Df(2L)BSC323. Isolation and characterization of Df(2L)BSC323. [FBrf0200221]
FlyBase analysis	Millburn et al., 2012.8.1, Lines in aberration datasets (files on ftp site). Lines in aberration datasets (files on ftp site). [FBrf0219195] FlyBase Curators, 2008, Release 5 estimated cytology of aberrations with sequence-defined breakpoints. Release 5 estimated cytology of aberrations with sequence-defined breakpoints. [FBrf0206255] FlyBase, 2007, En masse symbol-based assigment of Aberration Class with respect to wild type. En masse symbol-based assigment of Aberration Class with respect to wild type. [FBrf0191808]