[96A13-96A13];[96A22-96A22];
A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
96A13;96A22
Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.
Inferred to overlap with: Df(3R)Exel6200.
Homozygous third instar larvae show a significant increase in bouton size at the neuromuscular junction compared to controls.
The presence of P+PBac{XP5.RB3}BSC397 was verified using the PCR methods and primers described in FBrf0175003, with the
substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the
RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the
Supplementary Methods.
The cytological breakpoints of Df(3R)BSC397 predicted from the Release 5 genomic coordinates of the progenitor PBac{RB}e01519 and P{XP}d06348 transposable element insertion sites are 96A13;96A22.