[10F7-10F7];[11A11-11A11];
A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
10F7;11A11
Breakpoint based on release 3 sequence coordinate from Thibault et al., 2004, Supplementary Table 2 (FBrf0174227) or 3 (FBrf0174228), converted to release 5 coordinate.
Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.
The presence of P+PBac{XP5.WH5}BSC543 was verified using the PCR methods and primers described in FBrf0175003.
Exelixis, Inc. determined the insertion site of P{XP}d09482 to be Release 3 genomic coordinate 11651944 on the X chromosome. This corresponds to 10F7 on the Release 3 and Release 5 genome maps. The predicted position of PBac{WH}CG15731f05765 on the Release 5 map is 11A11. Consequently, the cytological breakpoints of Df(1)BSC543 are predicted to be 10F7;11A11.