[72D9-72D9];[72E1-72E1];
A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
72D9;72E1
Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.
Df(3L)Exel6128/Df(3L)BSC560 flies, derived from heterozygous parents, are viable, fertile and have no discernable phenotypes despite the fact these deficiencies overlap by 55 kb of genomic DNA. However, when these flies are inbred for several generations, their progeny often have thin bristles and etched abdominal tergites. These phenotypes are not rescued by either of two paternally inherited duplications (Ts(YSt;3Lt)ST1 or Dp(3;Y)L131-D3), indicating that the phenotypes are not caused by deleting the 55 kb genomic region, but by a maternal-effect mutation somewhere else in the genome.
Inferred to overlap with: Df(3L)ED220.
Inferred to overlap with: Df(3L)BSC443.
The presence of P+PBac{XP5.RB3}BSC560 was verified using the PCR methods and primers described in FBrf0175003, with the substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the Supplementary Methods.
The cytological breakpoints of Df(3L)BSC560 predicted from the Release 5 genomic coordinates of the progenitor P{XP}d02326 and PBac{RB}Nplp3e01799 transposable element insertions sites are 72D9;72E1.