[59B1-59B1];[60F5-60F5];
A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
59B1;60F5
Breakpoint based on release 3 sequence coordinate from Thibault et al., 2004, Supplementary Table 3 (FBrf0174228), converted to release 5 coordinate.
Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.
Inferred to overlap with: Df(2R)BSC863.
Inferred to overlap with: Df(2R)BSC864.
Inferred to overlap with: Df(2R)BSC865.
The presence of P+PBac{XP5.RB3}BSC599 was verified using the PCR methods and primers described in FBrf0175003 with the substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in FBrf0174229.
The cytological breakpoints of Df(2R)BSC599 predicted from the Release 5 genomic coordinates of the progenitor insertions are 59B1;59B3.