A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.
The presence of P+PBac{XP5.RB3}BSC658 was verified using the PCR methods and primers described in FBrf0175003 with the substitution of the primer 5'-GCTTCTAAACGCTTACGCATAAACGATG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in FBrf0174229.
The cytological breakpoints of Df(1)BSC658 predicted from the Release 5 genomic coordinates of the progenitor PBac{RB}CG1657e02476 and P{XP}rho-4d01806 insertion sites are 10B3;10C10.