A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
Breakpoint based on progenitor insertion release 3 sequence coordinate 3L:12039369 , per R. Hoskins (Gene Disruption Project) personal communication, mapped forward to release 5.
Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.
Df(3L)BSC727/+, hemo-A4/hemo-A4 mutant hemocytes do not exhibit a significant endolysosome phenotype.
The presence of P+PBac{XP5.RB3}BSC727 was verified using the PCR methods and primers described in FBrf0175003 with the substitution of the primer 5'-GCTTCTAAACGCTTACGCATAAACGATG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in FBrf0174229.
The cytological breakpoints of Df(3L)BSC727 predicted from the Release 5 genomic coordinates of the progenitor PBac{RB}e04464 and P{XP}d03723 insertion sites are 68D3;68F2.