A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
The presence of P+PBac{XP5.RB3}BSC788 was verified using the PCR methods and primers described in FBrf0175003 with the substitution of the primer 5’-GCTTCTAAACGCTTACGCATAAACGATG-3’ for the RB3’ plus or RB3’ minus primer in the Hybrid PCR protocol in the Supplementary Methods.
To confirm the presence of the deficiency, we showed that a 520 base pair fragment spanning the P{XP}d03120 (FBti0068833) insertion site could not be amplified from Df(3R)BSC788/P{XP}d03120 flies using the primers 5’-GGTGTGGTGGGGGCGAGTGAACCGC-3’ and 5’-CGATGAAAACGAATCCACGAATCGC-3’.
The breakpoints of Df(3R)BSC788 predicted from the Release 5 genomic coordinates of the progenitor PBac{RB}CG4553e02716 and P{XP}msid08770 transposable element insertion sites are 3R:21302593 ;21368879 and the cytological breakpoints predicted from these coordinates are 96E1;96E3.