A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
Lethal in combination with Df(2R)BSC786.
The presence of P+PBac{XP5.RB3}BSC802 was verified using the PCR methods and primers described in FBrf0175003 with the substitution of the primer 5’-GCTTCTAAACGCTTACGCATAAACGATG-3’ for the RB3’ plus or RB3’ minus primer in the Hybrid PCR protocol in FBrf0174229.
The breakpoints of Df(2R)BSC802 predicted from the Release 5 genomic coordinates of the progenitor P{XP}d10791 and PBac{RB}CG2921e02644 insertion sites are 2R:17759404 ;17963253 and the cytological breakpoints predicted from these coordinates are 58A3;58B3. The presence of a deletion was confirmed cytologically, though the breakpoints were not analyzed in detail.