A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
Lethal in combination with Df(2R)Exel6071.
Lethal in combination with Df(2R)Pu-D17.
Inferred to overlap with: Df(2R)BSC462.
The presence of P+PBac{XP5.RB3}BSC814 was verified using the PCR methods and primers described in FBrf0175003 with the substitution of the primer 5’-GCTTCTAAACGCTTACGCATAAACGATG-3’ for the RB3’ plus or RB3’ minus primer in the Hybrid PCR protocol in FBrf0174229.
The breakpoints of Df(2R)BSC814 predicted from the Release 5 genomic coordinates of the progenitor P{XP}d11699 and PBac{RB}e00751 insertion sites are 2R:16862884 ;16975752 and the cytological breakpoints predicted from these coordinates are 57B5;57B19.