A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
The presence of P+PBac{XP5.RB3}BSC844 was verified using the PCR methods and primers described in FBrf0175003 with the substitution of the primer 5'-GCTTCTAAACGCTTACGCATAAACGATG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the FBrf0174229.
The breakpoints of Df(3L)BSC844 predicted from the Release 5 genomic coordinates of the progenitor PBac{RB}CG6885e01335 and P{XP}d08572 transposable element insertion sites are 3L:18737336 ;18891426 and the cytological breakpoints predicted from these coordinates are 75D8;75E4.