A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
The presence of the deficiency was confirmed by showing that a 367 base pair fragment spanning the P{SUPor-P}CG12703KG08105 insertion site could not be amplified from Df(1)BSC871/P{SUPor-P}CG12703KG08105 flies using primers 5'-GTGATAACTTGTTCGATAGCTCTTCG-3' and 5'-CTTCCTGTACTACAACTGGTGGTGG-3'. Also, we showed that a 758 base pair fragment spanning the P{EPgy2}meso18EEY07842 insertion site could not be amplified from Df(1)BSC871/P{EPgy2}meso18EEY07842 flies using primers 5'-CGTCTAGAGCGTGGCGATTGTGCG-3' and 5'-GGAATCTCAGGACGGCGGACTACGG-3'.
