A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
The presence of P+PBac{XP5.RB3}BSC885 was verified using the PCR methods and primers described in FBrf0175003 with the substitution of the primer 5’-GCTTCTAAACGCTTACGCATAAACGATG-3’ for the RB3’ plus or RB3’ minus primer in the Hybrid PCR protocol in the Supplementary Methods.
To confirm the presence of Df(2R)BSC885, a 715 base pair fragment spanning the PBac{PB}CG30393[c05001] (FBti0041196) insertion site can not be amplified from Df(2R)BSC822/PBac{PB}CG30393[c05001] flies using primers 5’-CGCATGTTGTGGATGACCCAACC-3’ and 5’-GCGAGACAACCATCACATCAGTCCG-3’.
The breakpoints of Df(2R)BSC885 predicted from the Release 5 genomic coordinates of the progenitor P{XP}d03020 and PBac{RB}CG42672e00881 transposable element insertion sites are 2R:17102728 ;17189303 and the cytological breakpoints predicted from these coordinates are 57D2;57D10.