46kb duplication of sequence covering the entire Enhancer of split complex has been inserted into the M{3xP3-RFP.attP}ZH-51D docking site on the second chromosome using the P"["acman"]" system.
39% of embryos homozygous for the Df(3L)Brd-C1 and Df(3R)E(spl)δ-6 deletions and carrying one copy of Dp(3;2)E(spl)δ-8 fail to hatch and only 2% reach pupal stages.
Df(3R)E(spl)δ-6/Dp(3;2)E(spl)δ-8 embryos are able to hatch.