A TI{KozakGAL4} DNA cassette has been inserted into CG13531, replacing the coding sequence (coordinates of deleted sequence are 2R:22847904..22853725 , release 6 genome). This results in a simultaneous knock-out of CG13531 plus a knock-in of GAL4 that is expected to be expressed under the control of the endogenous regulatory sequences of CG13531 (predicted to gene trap all annotated transcripts of the gene). The deletion also removes part of CG3649. The TI{KozakGAL4} cassette was inserted via the CRISPR/Cas-9 drop-in technique, using a dsDNA donor vector with homology arms of length 200bp. The sgRNA sequences used to target the gene were: GGAGTTCTTGGAATGTTCTGCGG and AAAACAGTTTAAAATCACAATGG.