A TI{KozakGAL4} DNA cassette has been inserted into CG15403, replacing the coding sequence (coordinates of deleted sequence are 2L:3128669..3129462 , release 6 genome). This results in a simultaneous knock-out of CG15403 plus a knock-in of GAL4 that is expected to be expressed under the control of the endogenous regulatory sequences of CG15403 (predicted to gene trap all annotated transcripts of the gene). The deletion is also within a coding intron of the toc gene. The TI{KozakGAL4} cassette was inserted via the CRISPR/Cas-9 drop-in technique, using a dsDNA donor vector with homology arms of length 200bp. The sgRNA sequences used to target the gene were: CTTCGTTCATAAGTCATTCAGGG and AATTGAAAGCGTAGATCTGCAGG.