A TI{KozakGAL4} DNA cassette has been inserted into Nepl18, replacing the coding sequence (coordinates of deleted sequence are 3R:28875633..28877707 , release 6 genome). This results in a simultaneous knock-out of Nepl18 plus a knock-in of GAL4 that is expected to be expressed under the control of the endogenous regulatory sequences of Nepl18 (predicted to gene trap all annotated transcripts of the gene). The deletion also removes part of CG33203 (as Nepl18 is nested within an intron of CG33203). The TI{KozakGAL4} cassette was inserted via the CRISPR/Cas-9 drop-in technique, using a dsDNA donor vector with homology arms of length 200bp. The sgRNA sequences used to target the gene were: AAAATTAGTTGAGTTTAATTAGG and AAAAAACGAATGAACTATATAGG.