FB2025_01 , released February 20, 2025
Aberration: Dmel\Df(3R)CRIMIC-CR71235
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General Information
Symbol
Df(3R)CRIMIC-CR71235
Species
D. melanogaster
Name
FlyBase ID
FBab0049445
Feature type
Computed Breakpoints include
Sequence coordinates
3R:21,159,367..21,159,367 (Df(3R)CRIMIC-CR71235:bk1)
3R:21,161,071..21,161,071 (Df(3R)CRIMIC-CR71235:bk2)
Member of large scale dataset(s)
Nature of Aberration
Cytological Order
Progenitor
Class of aberration (relative to wild type)
Class of aberration (relative to progenitor)
Breakpoints
Carries alleles
Formalized genetic data
Genetic mapping information
Comments

A TI{KozakGAL4} DNA cassette has been inserted into cDIP, replacing the coding sequence (coordinates of deleted sequence are 3R:21159367..21161071 , release 6 genome). This results in a simultaneous knock-out of cDIP plus a knock-in of GAL4 that is expected to be expressed under the control of the endogenous regulatory sequences of cDIP (predicted to gene trap all annotated transcripts of the gene). The insertion is also within SNF4Aγ (as cDIP is nested within an intron of SNF4Aγ). The TI{KozakGAL4} cassette was inserted via the CRISPR/Cas-9 drop-in technique, using a dsDNA donor vector with homology arms of length 200bp. The sgRNA sequences used to target the gene were: TTCAGGTCGAACGTGAATCGTGG and TGTAGTGATCACATAAAAAATGG.

Comments on Cytology
Sequence Crossreferences
DNA sequence
Protein sequence
Gene Deletion and Duplication Data
Genes Deleted / Disrupted
Complementation Data
Completely deleted / disrupted
Partially deleted / disrupted
Molecular Data
Completely deleted
Genes NOT Deleted / Disrupted
Complementation Data
 
Molecular Data
 
Genes Duplicated
Complementation Data
Completely duplicated
Partially duplicated
Molecular Data
Completely duplicated
Partially duplicated
Genes NOT Duplicated
Complementation Data
 
Molecular Data
 
Affected Genes Inferred by Location (2)
Phenotypic Data
In combination with other aberrations
NOT in combination with other aberrations
Stocks (1)
Notes on Origin
Discoverer
 
Balancer / Genotype Variants of the Aberration
 
Separable Components
 
Other Comments
 
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Df(3R)CRIMIC-CR71235
Name Synonyms
Secondary FlyBase IDs
    References (1)