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General Information
Symbol
Dmel\βTub85D6
Species
D. melanogaster
Name
FlyBase ID
FBal0000027
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
B2t6, β2t6
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

G9409886A

Reported nucleotide change:

G?A

Amino acid change:

G56D | betaTub85D-PA

Reported amino acid change:

G56D

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid substitution within an internal cluster of variable amino acids that has been identified as an isotype-defining domain in vertebrate β-tubulins. Amino acid replacement: G56D. Nucleotide substitution: G?A.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

In embryos homozygous for βTub85D6, the distal tip of the salivary gland turns as in wild-type by stage 12. By stage 14, however, only the distal half of the mutant salivary glands are turned posteriorly.

Homozygous βTub85D6 mutant embryos display defects in fusion competent myoblast migration at stage 14.

Trans-heterozygous stage 13 βTub85Dn/βTub85D6 embryos display salivary gland migration defects.

Stage 13 βTub85D6/Df(3R)by10 embryos display salivary gland migration defects.

Misspecification of the morphology of the doublet of the sperm tail axoneme.

Affects microtubule function during spermatogenesis.

βTub85D6/βTub85Dn males are sterile. Chromosome movement during meiosis appears normal in homozygous males, and each nucleus is usually paired with a single, normal-sized mitochondrial derivative in the developing spermatids. Nuclear shaping occurs normally and nuclear alignment occurs relatively normally during spermatogenesis. Mature axonemes appear abnormal, with a core of densely staining material present in the A subfibres of the outer doublet microtubules, in addition to the core of densely staining material that is normally present in the central pair and accessory tubules. Occasionally, filled outer doublet B tubules are also seen.

Young axonemes in the spermatids of homozygous males look morphologically normal. Mature axonemes have a subtle defect - the outer doublet microtubules fill in with the densely staining material normally found only in the central pair and accessory tubules. Nuclear shaping is normal.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

βTub85D6 heterozygotes fail to rescue the male sterile phenotype of βTub60D::βTub85Dstar; no sperm is present in the seminal vesicles. βTub60D::βTub85Dstar and βTub85D6 isoforms disrupt a common aspect of axoneme assembly.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

Acidic electrophoretic variant. The protein encoded by βTub85D7 is stable and capable of forming an αβ tubulin heterodimer.

Class II allele. P{β2β3*-pW8} and βTub85D6 isoforms disrupt a common aspect of axoneme assembly.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (8)