In embryos homozygous for βTub85D6, the distal tip of the salivary gland turns as in wild-type by stage 12. By stage 14, however, only the distal half of the mutant salivary glands are turned posteriorly.
Homozygous βTub85D6 mutant embryos display defects in fusion competent myoblast migration at stage 14.
Trans-heterozygous stage 13 βTub85Dn/βTub85D6 embryos display salivary gland migration defects.
Stage 13 βTub85D6/Df(3R)by10 embryos display salivary gland migration defects.
Misspecification of the morphology of the doublet of the sperm tail axoneme.
Affects microtubule function during spermatogenesis.
βTub85D6/βTub85Dn males are sterile. Chromosome movement during meiosis appears normal in homozygous males, and each nucleus is usually paired with a single, normal-sized mitochondrial derivative in the developing spermatids. Nuclear shaping occurs normally and nuclear alignment occurs relatively normally during spermatogenesis. Mature axonemes appear abnormal, with a core of densely staining material present in the A subfibres of the outer doublet microtubules, in addition to the core of densely staining material that is normally present in the central pair and accessory tubules. Occasionally, filled outer doublet B tubules are also seen.
Young axonemes in the spermatids of homozygous males look morphologically normal. Mature axonemes have a subtle defect - the outer doublet microtubules fill in with the densely staining material normally found only in the central pair and accessory tubules. Nuclear shaping is normal.