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General Information
Symbol
Dmel\βTub85D7
Species
D. melanogaster
Name
FlyBase ID
FBal0000028
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
B2t7
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

G9409713A

Reported nucleotide change:

G?A

Amino acid change:

D114N | betaTub85D-PA

Reported amino acid change:

D114N

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: D114N. Nucleotide substitution: GAT to AAT.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Affects microtubule function during spermatogenesis.

Chromosome movement during meiosis usually appears normal in homozygous males, but failure of cytokinesis after meiosis I or II is frequent, with two or four nuclei found associated with a single, abnormally large mitochondrial derivative in the developing spermatids. Smaller mitochondrial derivatives than normal are also seen. Nuclear shaping during spermatogenesis occurs normally, but nuclear alignment is defective. Spermatid cysts often contain fewer axonemes than normal, and the axonemes generally lack one or both central pair microtubules. The axonemes are shorter than normal. Broken axonemes or axoneme fragments are common. Some spermatid cyst elongation takes place. Chromosome segregation during meiosis is sometimes abnormal in βTub85D7/βTub85Dn males. βTub85D7/βTub85Dn males are sterile.

Although relatively well organised near the basal body, the axonemes in the spermatids of homozygous males are abnormally short, appear to fray apart as the distance from the basal body exceeds more than a few μm and lack one or both central pair microtubules. Occasional failure of cytokinesis after meiosis I or II is seen. Nuclear shaping is normal.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement
Reference
Other
Statement
Reference
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

βTub85D7 heterozygotes partially rescue the male sterile phenotype of βTub60D::βTub85Dstar, some motile sperm is present in the seminal vesicles.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Basic electrophoretic variant. The protein encoded by βTub85D7 is stable and capable of forming an αβ tubulin heterodimer.

Class II allele.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (7)