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General Information
Symbol
Dmel\βTub85D8
Species
D. melanogaster
Name
FlyBase ID
FBal0000029
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
B2t8
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

G9409132A

Reported nucleotide change:

G?A

Amino acid change:

E288K | betaTub85D-PA

Reported amino acid change:

E288K

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: E288K. Nucleotide substitution: GAG to AAG.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Heterozygous males have significantly reduced fertility, although they do produce progeny. Chromosome segregation and cytokinesis during meiosis are abnormal in homozygous males. Chromosomes fail to move to the poles and remain scattered during meiosis I and II, although some separation of the chromosomes does occur. Early spermatids typically have multiple nuclei of various sizes, often associated with large mitochondrial derivatives. Nuclear shaping during spermatogenesis does not occur and nuclear alignment is defective. Clusters of abnormal microtubules are present in place of axonemes. In late spermatids these structures fill with the dense core material characteristic of wild-type central pair and accessory microtubules. Spermatid cyst elongation is very poor. Mitotic division before meiosis appears normal.

Flagellar elongation is extremely defective in homozygous males. The developing spermatids contain a variety of aberrant microtubule structures, the most common of which are S-shaped microtubules. Closed microtubules and outer doublet-type structures are rarely seen. S-shaped microtubules are seen in both the astral and central spindle region of the meiotic spindle, in the place of axonemes in developing spermatid bundles and next to the nucleus during nuclear shaping. Meiosis is defective. Nuclear shaping is defective; although a few nuclei begin to flatten, most remain large and round.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

βTub85D8 heterozygotes can fully rescue the male sterile phenotype of βTub60D::βTub85Dstar; a large mass of motile sperm, equivalent to that in wild type males, is present in the seminal vesicles.

Mutant males carrying one copy of αTub84B7.tHa and heterozygous for βTub85D8 display reduced fertility in mating tests; the aberrant protein from βTub85D8 retains a residual function.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Encodes an electrophoretic variant protein that is shifted two charge units towards the basic direction compared to wild-type. The protein is stable and capable of forming an αβ tubulin heterodimer.

Class II allele.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (9)