FlyBase Allele Report: Dmel\Antp[25]

General Information

Symbol

Dmel\Antp²⁵

Species

D. melanogaster

Name

FlyBase ID

FBal0000566

Feature type

allele

Associated gene

Dmel\Antp

Associated Insertion(s)

Carried in Construct

Also Known As

Antp^W10, Antp^RW10, Antennapedia²⁵

Key Links

Allele class

amorphic allele - genetic evidence

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(McCormick et al., 1995, Gould and White, 1992, Burgess and Duncan, 1990, Mann and Hogness, 1990, Reuter et al., 1990, Abbott and Kaufman, 1986)

loss of function allele

(Roy et al., 1997, Heuer and Kaufman, 1992)

Mutagen

ethyl methanesulfonate

(Pattatucci et al., 1991, Lewis et al., 1980)

Progenitor genotype

Cytology

Polytene chromosomes normal.

(Pattatucci et al., 1991)

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

antenna | ectopic | somatic clone

(Abbott and Kaufman, 1986)

embryonic midgut constriction 1

(Roy et al., 1997, Reuter and Scott, 1990)

external sensory organ & thorax

(Heuer and Kaufman, 1992)

femur | somatic clone

(Burgess and Duncan, 1990)

ganglion mother cell | embryonic stage | increased number

(Tsuji et al., 2008)

larval EL neuron | embryonic stage | increased number

(Tsuji et al., 2008)

leg | somatic clone

(Abbott and Kaufman, 1986)

mesothoracic dorsal triscolopidial chordotonal organ dch3

(Heuer and Kaufman, 1992)

mesothoracic leg disc | somatic clone

(Duncan et al., 2010)

mesothoracic segment | somatic clone

(Abbott and Kaufman, 1986)

metatarsus | proximal | somatic clone

(Burgess and Duncan, 1990)

metathoracic dorsal triscolopidial chordotonal organ dch3

(Heuer and Kaufman, 1992)

neuroblast NB3-3 | embryonic stage

(Tsuji et al., 2008)

posterior signaling center of lymph gland primary lobe (with Antp¹⁷)

(Tokusumi et al., 2010)

somatic muscle & mesothoracic segment

(Roy et al., 1997)

somatic muscle & metathoracic segment

(Roy et al., 1997)

tibia | somatic clone

(Burgess and Duncan, 1990)

Detailed Description

Statement

Reference

Homozygous clones in second leg discs fall into two categories; those that are well integrated into the disc epithelium and whose borders are interdigitated with their wild-type neighbours and those that are rounded up and have smooth borders. The interdigitated cones occur in all regions of the leg disc and appear to develop completely normally. The rounded up clones often have borders that coincide with novel folds in the disc. These rounded up clones almost always show some connection to the proximal ring that co-expresses Dll, hth and exd and are transformed to antenna (as assayed by marker expression).

(Duncan et al., 2010)

The posterior signaling center is absent in Antp²⁵/Antp¹⁷ mutant larvae.

(Tokusumi et al., 2010)

In Antp²⁵ embryos, thoracic neuroblast 3-3 (NB3-3T) fails to enter quiescence during mid-embryogenesis. Correspondingly, NB3-3T generates an increased number of GMCs and EL neurons compared to wild type.

(Tsuji et al., 2008)

Single cell class IV dendrite arborisation (da) neuron clones that are homozygous for Antp²⁵ show no significant defects in dendrite development.

(Parrish et al., 2007)

Loss of Antp function in Antp²⁵ mutants does not affect the NB6-4t lineage in any of the thoracic segments.

(Berger et al., 2005)

Late homozygous embryos have normal oenocyte clusters.

(Brodu et al., 2002)

Thoracic BrdU incorporation associated with ventrolateral/lateral neuroblasts is normal in homozygous embryos. However, additional staining is detected in ventral positions.

(Prokop et al., 1998)

Severe disorganisation of T2 and T3 muscle pattern and absence of the first midgut constriction.

(Roy et al., 1997)

Expression of nub in endoderm is wild type.

(Affolter et al., 1993)

Staining with antibodies reveals that Antp²⁵ is necessary for many thoracic es organs, it is required for lch3 axonal projection and contributes to ch organs in T2 and T3.

(Heuer and Kaufman, 1992)

No salivary gland defects.

(Panzer et al., 1992)

Homozygous clones in the leg show deletions of structures from the femur, tibia and proximal part of the basitarsus.

(Burgess and Duncan, 1990)

Hemizygotes lack the anterior constriction of the midgut. In hemizygotes Scr expression is very much reduced.

(Reuter and Scott, 1990)

X ray induced somatic clones homozygous for Antp²⁵ demonstrate that loss of Antp function results in the development of only a restricted portion of the thorax and mesothoracic legs are transformed to an antennal identity.

(Abbott and Kaufman, 1986)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Antp²⁵, ct^C145 has visible phenotype

(Johnston et al., 1998)

Antp²⁵, ct^L188 has visible phenotype

(Johnston et al., 1998)

Phenotype Manifest In

Suppressed by

Statement

Reference

Antp²⁵ has prothoracic ventral denticle belt phenotype, suppressible by Scer\GAL4^arm.PS/Ubx^Ia.VP16.UAS

(Li and McGinnis, 1999)

Antp²⁵ has embryonic head segment phenotype, suppressible by Scer\GAL4^arm.PS/Ubx^Ia.VP16.UAS

(Li and McGinnis, 1999)

Other

Statement

Reference

Antp²⁵, Ubx¹ has muscle cell of mesothoracic dorsal acute muscle 3 phenotype

(Enriquez et al., 2010)

Antp²⁵, Ubx¹ has muscle cell of metathoracic dorsal acute muscle 3 phenotype

(Enriquez et al., 2010)

Antp²⁵, Ubx¹ has muscle cell of abdominal 2 dorsal acute muscle 3 phenotype

(Enriquez et al., 2010)

Antp²⁵, Ubx¹ has muscle cell of abdominal 1 dorsal acute muscle 3 phenotype

(Enriquez et al., 2010)

Antp²⁵, ct^C145 has humeral bristle phenotype

(Johnston et al., 1998)

Antp²⁵, ct^C145 has pronotum phenotype

(Johnston et al., 1998)

Antp²⁵, ct^C145 has postpronotum phenotype

(Johnston et al., 1998)

Antp²⁵, ct^L188 has humeral bristle phenotype

(Johnston et al., 1998)

Antp²⁵, ct^L188 has pronotum phenotype

(Johnston et al., 1998)

Antp²⁵, ct^L188 has postpronotum phenotype

(Johnston et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

Antp²⁵, Ubx¹ double mutant embryos lack a DA3 muscle in all thoracic and abdominal A1-A2 segments.

(Enriquez et al., 2010)

Scr⁴ Antp²⁵ embryos show a wild-type oenocyte pattern.

(Brodu et al., 2002)

Ubx^{Ia.Scer\UAS.T:Hsim\VP16} driven by a weak expressing line of Scer\GAL4^arm.PS (arm-GAL^4r), in a Antp²⁵ mutant background, displays a normal Antp loss of function phenotype. When driven by a strong expressing line of Scer\GAL4^arm.PS (arm-GAL⁴), the Antp²⁵ is rescued almost to wild type; T2 and T3 are rescued to their normal appearances, though some head segments develop small patches of T2-like denticles.

(Li and McGinnis, 1999)

In heterozygous combination with ct^L188 or ct^C145, ectopic structures project (usually unilaterally) from the position of the anterior spiracle on the dorsal prothorax, and are associated with malformation of absence of the two macrochaetaes on the humeral callus. The phenotype is incompletely penetrant and not as severe as for Antp¹¹.

(Johnston et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Antp²⁵ is partially rescued by Antp^UAS.cCa/Scer\GAL4^e22c

(Roy et al., 1997)

Comments

Scer\GAL4^e22c-mediated expression of Antp^Scer\UAS.cCa rescues the muscle defects in T2.

(Roy et al., 1997)

Images (0)

Mutant

Wild-type

Stocks (1)

Kyoto

107167

Dp(3;1)P115; Df(3R)P9, st¹ in¹ kni^ri-1 Pc³ Scr⁴ Antp²⁵/TM3, Sb¹

Notes on Origin

Discoverer

Wakimoto.

Induced on: red¹ e¹.

(Johnston et al., 1998)

Comments

Reduced levels of tsh transcripts are found in parasegments 4 and 5.

(Roder et al., 1992)

The A and B glial cells do not show a pattern defect.

(Prokop and Technau, 1994)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

Antp^RW10

(Brodu et al., 2002, Edgar et al., 1994)

Antp^W10

(Capovilla et al., 2001, Maurel-Zaffran et al., 1999, Prokop et al., 1998, Graba et al., 1995, Graba et al., 1994, Mathies et al., 1994, Prokop and Technau, 1994, Rauskolb and Wieschaus, 1994, Gould and White, 1992, Graba et al., 1992, Pattatucci et al., 1991, Burgess and Duncan, 1990, Peifer and Wieschaus, 1990)

Antp^rW10

(Abbott and Kaufman, 1986)

Antp^w10

(Bilder and Scott, 1998, Roy et al., 1997, Haerry and Gehring, 1996, McCormick et al., 1995, Yamaguchi et al., 1995, Appel and Sakonju, 1993, Chan and Mann, 1993, Panzer et al., 1992, Roder et al., 1992, Mann and Hogness, 1990, Reuter et al., 1990, Riley et al., 1987)

AntpW10

(Chauvet et al., 2000)

EfW10

(Lewis et al., 1980)

antp²⁵

(Lacin and Truman, 2016, Zacharioudaki et al., 2016)

Name Synonyms

Antennapedia25

(Kannan et al., 2010, Sanders et al., 2008)

Secondary FlyBase IDs

FBal0028972

References (69)

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